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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 01, 2023 |
| Title |
Linking erythropoietin to regulatory T-cell-dependent allograft survival through myeloid cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Erythropoietin (EPO) has multiple functions beyond stimulating erythrocytosis, including modulation of T cell immunity. Effects EPO in transplantation and associated mechanisms remain incompletely understood. We analyzed outcomes and performed cellular and molecular mechanistic assessments in murine wild type (WT) and conditional EPO receptor (EPOR) knockout recipients of cardiac allografts. In translational studies, we tested EPO’s effects in a non-human primate system.
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| Overall design |
B6 mice were immunized with 20x106 BALB/c splenocytes. Recipient mice were treated with CTLA4-Ig (150µg i.p. once on day 2) (Abatacept, Bristol-Myers Squibb, New York, NY) ± recombinant EPO (5000 U/kg i.p. daily) (epoetin alfa, Amgen, Thousand Oaks, CA). On day 7, splenic CD11b+ myeloid cells were isolated by positive selection (kit #18970, STEMCELL, Vancouver, BC, Canada). RNA was isolated using TRIzol (Thermo Fisher Scientific, Waltham, MA) and cleaned using RNeasy Mini Kit (Qiagen, Hilden, Germany). Gene expression profiling was then performed using the nCounter® Myeloid Innate Immunity Panel (NanoString Technologies, Seattle, WA).
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| Contributor(s) |
Horwitz JK, Yi Z, Zhang W, Heeger PS |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 23, 2022 |
| Last update date |
Mar 04, 2023 |
| Contact name |
Weijia Zhang |
| E-mail(s) |
[email protected]
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| Organization name |
Icahn School of Medicine at Mount Sinai
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| Department |
Renal
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| Lab |
Bioinfomatics
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| Street address |
1 Gustave L. Levy Pl
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL25266 |
NanoString Mouse nCounter Myeloid Innate Immunity Panel NS_Mm_Myeloid_V2.0 |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA819094 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE199270_RAW.tar |
160.0 Kb |
(http)(custom) |
TAR (of RCC) |
| Processed data included within Sample table |
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