|
| Status |
Public on Dec 10, 2009 |
| Title |
DUO1 ectopic expression |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The experiment is designed to examine changes in gene expression upon ectopic expression of the germ line transcription factor DUO1 in Arabidopsis. Plants were transformed with the vector pMDC7DUO1, allowing induction of DUO1 by treatment with estradiol. T2 seedlings were selected on agar plates containing hygromycin for 12 days before seedlings were transferred to plates containing either 2 µM 17beta-estradiol (induced), or control plates containing DMSO (non-induced). Plants were grown for 6, 12 or 24 hours before harvesting. RNA was extracted using the RNAeasy kit from Qiagen. Each sample consists of 20 seedlings combined and all treatments have been conducted in triplicate.
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| |
|
| Overall design |
18 samples were used in this experiment.
|
| |
|
| Contributor(s) |
Brownfield L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 02, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (18)
|
| GSM477967 |
Brownfield_1-1_6hr-not-induced_Rep1_ATH1 |
| GSM477968 |
Brownfield_1-2_6hr-not-induced_Rep2_ATH1 |
| GSM477969 |
Brownfield_1-3_6hr-not-induced_Rep3_ATH1 |
| GSM477970 |
Brownfield_1-4_6hr-induced_Rep1_ATH1 |
| GSM477971 |
Brownfield_1-5_6hr-induced_Rep2_ATH1 |
| GSM477972 |
Brownfield_1-6_6hr-induced_Rep3_ATH1 |
| GSM477973 |
Brownfield_1-7_12hr-not-induced_Rep1_ATH1 |
| GSM477974 |
Brownfield_1-8_12hr-not-induced_Rep2_ATH1 |
| GSM477975 |
Brownfield_1-9_12hr-not-induced_Rep3_ATH1 |
| GSM477976 |
Brownfield_1-10_12hr-induced_Rep1_ATH1 |
| GSM477977 |
Brownfield_1-11_12hr-induced_Rep2_ATH1 |
| GSM477978 |
Brownfield_1-12_12hr-induced_Rep3_ATH1 |
| GSM477979 |
Brownfield_1-13_24hr-not-induced_Rep1_ATH1 |
| GSM477980 |
Brownfield_1-14_24hr-not-induced_Rep2_ATH1 |
| GSM477981 |
Brownfield_1-15_24hr-not-induced_Rep3_ATH1 |
| GSM477982 |
Brownfield_1-16_24hr-induced_Rep1_ATH1 |
| GSM477983 |
Brownfield_1-17_24hr-induced_Rep2_ATH1 |
| GSM477984 |
Brownfield_1-18_24hr-induced_Rep3_ATH1 |
|
| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA120937 |
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