|
| Status |
Public on Dec 10, 2009 |
| Title |
Brownfield_1-15_24hr-not-induced_Rep3_ATH1 |
| Sample type |
RNA |
| |
|
| Source name |
pMDC7DUO1, not induced, 24hr
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
developmental stage: 1.04
tissue: whole plant
genetic background: Col-0
genetic variation: T-DNA mutation
duo1 induction: no
time: 24hr
|
| Growth protocol |
Seeds were sterilized by washing for 5 min with 70% (w/v) ethanol/0.05% (w/v) triton-X 100 and then 10 min with 100% ethanol. Approximately 200 dry seeds were then spread on a 14cm Petri dish containing 1 X Murashige and Skoog basal salts, 0.8% phytagar and 20 ug/ml hyromycin with no carbon source. Plates were placed at 4C for 3 days then plants grown under constant light at 22C for 12 days before treatment began.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy kit protocol
|
| Label |
biotin
|
| Label protocol |
Labelled using protocol described in manual "Affymetrix GeneChip Expression Analysis Technical Manual", One-Cycle target labelling
|
| |
|
| Hybridization protocol |
Biotin Labelled cRNA using "Affymetrix GeneChip Hybridization, Wash and Stain Kit", according to guidelines set out by Affymetrix, Santa Clara, CA
|
| Scan protocol |
Scanned according to guidelines set out by Affymetrix, Santa Clara, CA.
|
| Description |
3 days at 4C on agar plate. 70% Ethanol/0.05% Tritin X-100 for 5 min. 100% Ethanol for 10 min. Air dry before plating Phytagar Growth room 22C 95% Murashige & Skoog basal salt mixture.
|
| Data processing |
Raw data from the microarrays was normalized at probe-level using MAS5 algorithm. The detection calls (present, marginal, absent) for each probe set was obtained using the GCOS system
|
| |
|
| Submission date |
Dec 02, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
|
| Relations |
| Reanalyzed by |
GSE118579 |
