|
| Status |
Public on Feb 23, 2011 |
| Title |
Expression profile of ypr045cā mutant |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
We report a new protein complex with a role in transcription elongation that is formed by Ypr045c (Thp3) and the Csn12 component of the COP9-signalosome. Thp3-Csn12 is recruited to transcribed genes. Their mutations suppress the gene expression defects of mutants of the THO complex involved in mRNP biogenesis and export and show defects in mRNA accumulation. In vivo transcription elongation impairment of thp3ā mutants is shown by reduction of RNAPII recruitment throughout an active gene and in transcript run on analysis performed in G-less systems. This new complex establishes a novel link between transcription and mRNA processing.
|
| |
|
| Overall design |
Tthree repeats of the wild type control and three of the ypr045cā mutant (BY4741 background).
|
| |
|
| Contributor(s) |
Jimeno S, Aguilera A |
| Citation(s) |
21149575 |
| |
| Submission date |
Nov 12, 2009 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Sonia Jimeno |
| E-mail(s) |
[email protected]
|
| Phone |
0034954467728
|
| Organization name |
CABIMER-CSIC
|
| Department |
Molecular biology
|
| Lab |
BM3
|
| Street address |
Americo Vespucio s/n
|
| City |
Seville |
| State/province |
Seville |
| ZIP/Postal code |
41912 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA120661 |
Less...
More...