|
| Status |
Public on Feb 23, 2011 |
| Title |
ypr045c rep-1 |
| Sample type |
RNA |
| |
|
| Source name |
Saccharomyces cerevisiae, ypr045c deletion mutant
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype/variation: BY4741 ypr045c∆
|
| Growth protocol |
Exponencially growing cells in YPD rich media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
|
| Scan protocol |
Microarrays were scanned using GeneChip® Scanner 3000 7G
|
| Description |
no additional information
|
| Data processing |
Analysis of microarray data was carried out with DNA-Chip Analyzer (www.dchip.org;(Li and Wong 2001). Normalization of probe cell files and computation of expression values were performed using the Invariant Set Normalization method and the Model Based method, respectively (Li and Wong, 2001b).
|
| |
|
| Submission date |
Nov 12, 2009 |
| Last update date |
Feb 23, 2011 |
| Contact name |
Sonia Jimeno |
| E-mail(s) |
[email protected]
|
| Phone |
0034954467728
|
| Organization name |
CABIMER-CSIC
|
| Department |
Molecular biology
|
| Lab |
BM3
|
| Street address |
Americo Vespucio s/n
|
| City |
Seville |
| State/province |
Seville |
| ZIP/Postal code |
41912 |
| Country |
Spain |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE18994 |
Expression profile of ypr045c∆ mutant |
|
