|
| Status |
Public on May 31, 2011 |
| Title |
Delineation of EZH2 oncogenic functions in hepatocellular carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
The goal of this study was to delineate the important EZH2 direct target genes that mediate the oncogenic properties of EZH2 in HCC. The EZH2 direct target genes in two HCC cell lines were identified by chromatin immunoprecipitation microarray (ChIP-chip) analysis and later confirmed by independent ChIP-PCR. The functions of the target genes were further examined.
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| |
|
| Overall design |
Two human HCC cell lines, Huh7 and PLC/PRF/5 (PLC5), were grown in DMEM supplemented with 10% fetal bovine serum. ChIP assays were performed using anti-EZH2 antibody. The immunoprecipitated and input DNA were used to probe the Agilent human ChIP-chip arrays.
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| |
|
| Contributor(s) |
Cheng AS, Choy RK |
| Citation(s) |
21512140 |
| |
| Submission date |
Aug 20, 2009 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Richard KW CHOY |
| E-mail(s) |
[email protected]
|
| Phone |
852-26323099
|
| Fax |
852-26360008
|
| Organization name |
The Chinese University of Hong Kong
|
| Department |
Obstetrics & Gynaecology
|
| Street address |
1/F, Block E, Prince of Wales Hospital
|
| City |
Shatin |
| ZIP/Postal code |
SAR |
| Country |
Hong Kong |
| |
|
| Platforms (2) |
| GPL4124 |
Agilent-014706 Human Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 G4489A (Feature Number version) |
| GPL4125 |
Agilent-014707 Human Promoter ChIP-on-Chip Set 244K, Microarray 2 of 2 G4489A (Feature Number version) |
|
| Samples (8)
|
| GSM442662 |
Huh7 EZH2 ChIP-chip Replicate 1 Slide 1 |
| GSM442663 |
Huh7 EZH2 ChIP-chip Replicate 1 Slide 2 |
| GSM442664 |
Huh7 EZH2 ChIP-chip Replicate 2 Slide 1 |
| GSM442665 |
Huh7 EZH2 ChIP-chip Replicate 2 Slide 2 |
| GSM442666 |
PLC5 EZH2 ChIP-chip Replicate 1 Slide 1 |
| GSM442667 |
PLC5 EZH2 ChIP-chip Replicate 1 Slide 2 |
| GSM442668 |
PLC5 EZH2 ChIP-chip Replicate 2 Slide 1 |
| GSM442669 |
PLC5 EZH2 ChIP-chip Replicate 2 Slide 2 |
|
| Relations |
| BioProject |
PRJNA118385 |
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