|
| Status |
Public on Jul 15, 2009 |
| Title |
Trf4p in vivo crosslinking and ribonucleoprotein-immunopurification-chip analysis (X-RIP-Chip) |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
In vivo cross-linking and ribonucleoprotein-immunopurification experiments followed by microarray analysis of bound RNAs (X-RIP-chip). Cells expressing recombinant tandem-affinity purification (TAP)-tagged Trf4 protein were cross-linked with formaldehyde, and Trf4-containing ribonucleoprotein complexes were recovered by affinity selection on IgG-coupled beads (see linked protocol). As a control for non-specifically enriched RNAs, the same experiment was done with untagged WT cells and with cells expressing Fpr1-TAP, a peptidyl-prolyl-cis-trans-isomerase not expected to bind RNA.
Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.
Antigenic peptide used in IP: Protein A derivative
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| |
|
| Overall design |
Computed
|
| |
|
| Contributor(s) |
Gerber A |
| Citation(s) |
19593367 |
| |
| Submission date |
May 14, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Andre Gerber |
| E-mail(s) |
[email protected]
|
| Phone |
+41 44 6337378
|
| Fax |
+41 44 6337364
|
| Organization name |
Swiss Federal Institute of Technology
|
| Department |
Institute of Pharmaceutical Science
|
| Street address |
Wolfgang Pauli Str. 10, HCI H396
|
| City |
ETH Hoenggerberg, Zuerich |
| ZIP/Postal code |
ZH |
| Country |
Switzerland |
| |
|
| Platforms (2) |
| GPL8306 |
SMD Print_1381 Saccharomyces cerevisiae |
| GPL8555 |
SMD Print_1380 Saccharomyces cerevisiae |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE16107 |
trf4, trf5, and trf4-DADA mutant cells gene expression; Trf4p X-RIP-Chip |
|
| Relations |
| BioProject |
PRJNA122819 |
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