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| Status |
Public on Jan 13, 2022 |
| Title |
Proteasome Inhibition Targets the KMT2A Transcriptional Complex |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
We quantitatively evaluated H2Bub and H3K79me2 epigenetic marks genome wide in a KMT2A rearranged B lineage acute lymphoblastic leukemia cell line after treatment with bortezomib over a 6 hour time course by ChIP-Rx. We identified rapid and concordant depletion of these epigenetic marks within two hours. To look at gene expression changes, RNA sequencing was performed on KMT2A rearranged B lineage acute lymphoblastic leukemia patient samples in the presence and absence of bortezomib exposure.
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| Overall design |
Examination of 2 different histone modifications in the SEM cell line following in vitro exposure to bortezomib by ChIP with reference exogenous genome (ChIP-Rx) using Drosophila S2 cells to allow quantitation over the 6 hour time course. 5 patient samples were exposed to bortezomib or DMSO for 20 hours followed by RNA sequencing to look at changes to gene expression that correlate with these histone modifications.
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| Contributor(s) |
Gruber TA |
| Citation(s) |
36781850 |
| |
| Submission date |
Jun 19, 2020 |
| Last update date |
Feb 15, 2023 |
| Contact name |
Tanja A Gruber |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford University
|
| Department |
Pediatrics
|
| Street address |
1000 Welch Road Suite 300
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (26)
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| Relations |
| BioProject |
PRJNA640535 |
| SRA |
SRP267965 |
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