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| Status |
Public on Aug 10, 2009 |
| Title |
IL-4-primed bone marrow macrophages isolated from C57Bl6/J or Balb/cAnNCrl mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To compare the expression profile of differentiated mouse bone marrow macrophages (BMM) in response to IL-4, we have employed whole genome microarray expression profiling. For this purpose, bone marrow cells were isolated from 8 to 12 weeks old C57BL6/J and Balb/cAnNCrl mice and cultured in the presence of the macrophage colony-stimulating factor (M-CSF). After seven days of culture, IL-4 was added for 4 and 18 hours.
Keywords: Mice strain comparison; Gene expression profiling
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| Overall design |
IL-4 induced gene expression was investigated in mouse bone marrow macrophages (BMM) of C57BL6/J and Balb/cAnNCrl mice. Differentiated BMM were incubated with mouse recombinant IL-4 for 4 or 18 hours or without for 18 hours. Two independent experiments were performed at each time (mock, 4 and 18 hours) using different mice littermates for each experiment.
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| Contributor(s) |
El Chartouni C, Rehli M |
| Citation(s) |
19687144 |
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| Submission date |
Jan 29, 2009 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Carol El Chartouni |
| Organization name |
University Hospital Regensburg
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| Department |
Hematology and oncology
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| Lab |
PD.Dr. Rehli
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| Street address |
Franz-Josef-Strauß Allee 11
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| City |
Regensburg |
| ZIP/Postal code |
93053 |
| Country |
Germany |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA112305 |
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