|
| Status |
Public on Dec 03, 2019 |
| Title |
Functional interactions between Mi-2β and AP1 complexes control response and recovery from barrier disruption |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Keratinocytes respond to environmental signals by eliciting induction of genes that preserve skin’s integrity. Here we show that the transcriptional response to stress signaling is supported by short-lived epigenetic changes. Comparison of chromatin accessibility and transcriptional changes induced by barrier disruption or by loss of the nucleosome remodeler Mi-2β identified their striking convergence in mouse and human keratinocytes. Mi-2β directly repressed genes induced by barrier disruption by restricting AP1-enriched promoter-distal sites, occupied by Mi-2β and JUNB at steady state and by c-JUN after Mi-2β depletion or stress signaling. Barrier disruption led to a modest reduction in Mi-2β expression and a further selective reduction of Mi-2β localization at stress response genes possibly through competition with activated c-JUN. Consistent with a repressive role at stress response genes, genetic ablation of Mi-2β did not prevent re-establishment of barrier integrity but was required for return to homeostasis. Thus a competition between Mi-2β repressive and activating AP1 complexes may permit rapid transcriptional response to and resolution from stress signaling.
|
| |
|
| Overall design |
24 mRNA-seq, 8 ATAC-seq, 22 ChIP_seq samples
|
| |
|
| Contributor(s) |
Shibata S, Kashiwagi M |
| Citation(s) |
31834931 |
| |
| Submission date |
Oct 31, 2019 |
| Last update date |
Jun 11, 2020 |
| Contact name |
Katia Georgopoulos |
| E-mail(s) |
[email protected]
|
| Phone |
617-7264445
|
| Organization name |
Harvard Medical School
|
| Department |
CBRC
|
| Lab |
Georgopoulos
|
| Street address |
1st and 13th St
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL21697 |
NextSeq 550 (Homo sapiens) |
|
| Samples (53)
|
|
| Relations |
| BioProject |
PRJNA586885 |
| SRA |
SRP227878 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE139685_ATACpeaks_Mi2KOvsWT_MACS2.xls.gz |
693.5 Kb |
(ftp)(http) |
XLS |
| GSE139685_ATACpeaks_TS24hrvsWT_MACS2.xls.gz |
157.7 Kb |
(ftp)(http) |
XLS |
| GSE139685_ATACpeaks_TS6hrvsWT_MACS2.xls.gz |
240.1 Kb |
(ftp)(http) |
XLS |
| GSE139685_ATACpeaks_WTvsMi2KO_MACS2.xls.gz |
51.2 Kb |
(ftp)(http) |
XLS |
| GSE139685_ATACpeaks_WTvsTS24hr_MACS2.xls.gz |
1.7 Kb |
(ftp)(http) |
XLS |
| GSE139685_ATACpeaks_WTvsTS6hr_MACS2.xls.gz |
2.6 Kb |
(ftp)(http) |
XLS |
| GSE139685_HaCaT_Mi2ChIP.txt.gz |
221.2 Kb |
(ftp)(http) |
TXT |
| GSE139685_Human_RNAseq_DEseq2.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
| GSE139685_Mouse_TS_RNAseq_DEseq2.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
| GSE139685_hKRT_CTR_H3K27AcChIP.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
| GSE139685_hKRT_CTR_H3K4me3ChIP.txt.gz |
563.3 Kb |
(ftp)(http) |
TXT |
| GSE139685_hKRT_CTR_JUNBChIP.txt.gz |
181.8 Kb |
(ftp)(http) |
TXT |
| GSE139685_hKRT_CTR_Mi2ChIP_1.txt.gz |
260.9 Kb |
(ftp)(http) |
TXT |
| GSE139685_hKRT_CTR_Mi2ChIP_2.txt.gz |
436.7 Kb |
(ftp)(http) |
TXT |
| GSE139685_hKRT_CTR_RNApIIChIP.txt.gz |
835.9 Kb |
(ftp)(http) |
TXT |
| GSE139685_hKRT_CTR_cJUNChIP.txt.gz |
4.8 Kb |
(ftp)(http) |
TXT |
| GSE139685_hKRT_Mi2KD_H3K27AcChIP.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE139685_hKRT_Mi2KD_H3K4me3ChIP.txt.gz |
609.2 Kb |
(ftp)(http) |
TXT |
| GSE139685_hKRT_Mi2KD_RNApIIChIP.txt.gz |
941.1 Kb |
(ftp)(http) |
TXT |
| GSE139685_hKRT_Mi2KD_cJUNChIP.txt.gz |
313.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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