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| Status |
Public on May 01, 2019 |
| Title |
Single cell RNAseq of human TCRVdelta 1 and TCRVdelta 2 gammadelta T lymphocytes purified from healthy adults blood |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
γδ T lymphocytes represent ~1% of human PBMC and even more cells in most tissues of vertebrates. Although they have important anticancer functions, most current scRNA-Seq studies do not identify γδ T lymphocytes since their transcriptomes at the single cell level are unknown. Here we show that high resolution clustering of large scRNA-Seq data sets and a combination of gene signatures allow the specific detection of human γδ T lymphocytes and identification of their TCRVδ1 and TCRVδ2 subsets in large data sets from complex cell mixtures. In t-SNE plots from blood and tumor samples, the few γδ T lymphocytes appear collectively embedded between cytotoxic CD8 T and NK cells. Their TCRVδ1 and TCRVδ2 subsets form close yet distinct sub-clusters respectively neighbouring NK and CD8 T cells owing to expression of shared and distinct cytotoxic maturation genes. Similar pseudo-time maturation trajectories of TCRVδ1 and TCRVδ2 γδ T lymphocytes were discovered, unveiling in both subsets an unattended pool of TEMRA cells with preserved proliferative capacity, a finding confirmed by in vitro proliferation assays. Overall, the single cell transcriptomes of thousands of individual gd T lymphocytes from different CMV+ and CMV- donors reflect cytotoxic maturation stages driven by the immunological history of donors. This landmark study establishes the rationale for identification, subtyping and deep characterization of human γδ T lymphocytes in further scRNA-Seq studies of complex tissues in physiological and disease conditions.
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| Overall design |
6 samples of purified human gd T cells from 3 donors (CMV+ and CMV-). Cell-sorted TCRVdelta1 and TCRVdelta2 T lymphocytes from each donor PBMC.
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| Contributor(s) |
Fournié J |
| Citation(s) |
31118283 |
| |
| Submission date |
Mar 13, 2019 |
| Last update date |
Apr 18, 2020 |
| Contact name |
Jean Jacques FOURNIE |
| E-mail(s) |
[email protected]
|
| Phone |
+33619900602
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| Organization name |
INSERM
|
| Department |
oncology
|
| Lab |
Cancer Research Center of Toulouse
|
| Street address |
2 av Hubert Curien
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| City |
Toulouse |
| ZIP/Postal code |
31037 |
| Country |
France |
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| Platforms (2) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA526841 |
| SRA |
SRP188300 |
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