|
| Status |
Public on May 01, 2019 |
| Title |
Donor_1_d2 |
| Sample type |
SRA |
| |
|
| Source name |
purified Vgd2
|
| Organism |
Homo sapiens |
| Characteristics |
subject id: Donor_1
cell type: purified TCRVdelta 2 gammadelta T lymphocytes
cmv status: CMV+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sequencing library was prepared following the manufacturer’s instructions (10X Genomics), with 14 cycles used for cDNA amplification and 12 cycles for library amplification
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
Raw data were firstly demultiplexed using mkfastq application (Cell Ranger v1.2.1).
Fastq files were then run with count application (Cell Ranger v1.2.1) using default settings, which performs alignment (using STAR aligner), filtering and UMI counting. The UMI count table was used for further analysis.
Genome_build: Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include UMI numbers for all genes and single cells that passed filtering
|
| |
|
| Submission date |
Mar 13, 2019 |
| Last update date |
May 02, 2019 |
| Contact name |
Jean Jacques FOURNIE |
| E-mail(s) |
[email protected]
|
| Phone |
+33619900602
|
| Organization name |
INSERM
|
| Department |
oncology
|
| Lab |
Cancer Research Center of Toulouse
|
| Street address |
2 av Hubert Curien
|
| City |
Toulouse |
| ZIP/Postal code |
31037 |
| Country |
France |
| |
|
| Platform ID |
GPL21290 |
| Series (1) |
| GSE128223 |
Single cell RNAseq of human TCRVdelta 1 and TCRVdelta 2 gammadelta T lymphocytes purified from healthy adults blood |
|
| Relations |
| BioSample |
SAMN11115774 |
| SRA |
SRX5512674 |
