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| Status |
Public on Mar 11, 2024 |
| Title |
RNA levels in wild-type and HIF1α-mutant MCF7 cells in normoxia (21% O2), and in hypoxia (1% O2) for 3 h or 24 h |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the generation of CRISPR/Cas9-engineered MCF7 human breast cancer cells that lack functional HIF1α. We show that the HIF1α-dependent transcriptional response to hypoxia is severely impaired in HIF1α-mutant MCF7 cells.
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| |
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| Overall design |
RNA profiles of 2 cell lines (wild-type and HIF1α-mutant MCF7 cells ) in 3 conditions (grown at 21% oxygen, at 1% oxygen for 3 hours and at 1% oxygen for 24 hours) in triplicates, using Illumina HiSeq 2500
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| Web link |
https://doi.org/10.1038/s44318-024-00065-w
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| |
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| Contributor(s) |
Anastasiou D, Grimm F |
| Citation(s) |
38485816 |
| |
| Submission date |
Nov 01, 2018 |
| Last update date |
May 08, 2024 |
| Contact name |
Dimitrios Anastasiou |
| E-mail(s) |
[email protected]
|
| Organization name |
The Francis Crick Institute
|
| Department |
Cancer Metabolism Laboratory
|
| Street address |
1 Midland Road
|
| City |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA503341 |
| SRA |
SRP167432 |
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