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| Status |
Public on Aug 10, 2021 |
| Title |
Screening of Genes Related to Glucose and Lipid Metabolism in Non-alcoholic Fatty Liver Disease by Gene Microarray |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
We used gene expression microarray to explore the correlation between gene expression profiles and find out the target genes related to lipid and glucose metabolism in NAFLD. A rat model of NAFLD was established by feeding male rats with high-fat diet and Agilent DNA microarray was used to detect gene expression profiles of liver tissues. We explored differentially expressed genes and intersection of them by using Gene ontology (GO) and Pathway analyses. Then, target genes were verified by Real-time polymerase chain reaction (RT-PCR). There were 932 genes, including 783 up-regulated and 149 down-regulated, differed in expression compared to those of control rats. The up-regulated genes were involved in biosynthesis, cell development, cell differentiation and down-regulated genes mainly contributed to biological metabolic process and stress reaction. We identified genes involved in insulin signaling pathway, Notch signaling pathway, adipocytokin and linoleic acid metabolism to be closely related to liver fat content and insulin resistance. Among them, genes which supposed to connect glucose and lipid metabolism were screened (IGFBP7, Notch1, HMGCR and ACACB) and then validated by real-time PCR. These data provide innovative information on the whole genome expression profile due to high-fat diet feeding and bring important insights into the mechanism of NAFLD.
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| Overall design |
Liver tissues from male rats were distributed into two experimental groups: normal control group (Zheng) and NAFLD tissue group (N), then Agilent DNA microarray was used to detect gene expression profiles of liver tissues
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| Contributor(s) |
Li T, Lu X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 22, 2018 |
| Last update date |
Aug 11, 2021 |
| Contact name |
ting li |
| E-mail(s) |
[email protected]
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| Phone |
0086-18710708230
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| Organization name |
Xi’an Jiaotong University
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| Department |
Health Science Center
|
| Street address |
NO.76 Yanta West Road, Health Science Center, Xi’an Jiaotong University, Xi’an. China
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| City |
Xi’an. China |
| State/province |
Shaanxi |
| ZIP/Postal code |
710061 |
| Country |
China |
| |
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| Platforms (1) |
| GPL14746 |
Agilent-028282 Whole Rat Genome Microarray 4x44K v3 (Probe Name version) |
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| Samples (8)
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| GSM3350331 |
Liver tissue_14 weeks_high fat diet_rep1 |
| GSM3350332 |
Liver tissue_14 weeks_high fat diet_rep2 |
| GSM3350333 |
Liver tissue_14 weeks_high fat diet_rep3 |
| GSM3350334 |
Liver tissue_14 weeks_high fat diet_rep4 |
| GSM3350335 |
Liver tissue_14 weeks_high fat diet_rep5 |
| GSM3350336 |
Liver tissue_14 weeks_standard chow diet_rep1 |
| GSM3350337 |
Liver tissue_14 weeks_standard chow diet_rep2 |
| GSM3350338 |
Liver tissue_14 weeks_standard chow diet_rep3 |
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| Relations |
| BioProject |
PRJNA487162 |
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