|
| Status |
Public on Aug 13, 2018 |
| Title |
SIRT1 Activation Disrupts Maintenance of Myelodysplastic Syndrome Stem and Progenitor Cells by Restoring TET2 Function [hMeDIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Improved understanding of mechanisms regulating myelodysplastic syndrome (MDS) hematopoietic stem/progenitor cell (HSPC) growth and self-renewal is critical for developing MDS therapy. We revealed a novel regulatory axis that SIRT1-deficiency induced TET2 hyperacetylation promotes MDS HSPC functions, and provide an approach to target MDS HSPCs by activating SIRT1 deacetylase.
|
| |
|
| Overall design |
5-hydroxymethylcytosine (5hmC) modified DNA fragments from SIRT1 overexpressed or MOCK MDS-L cells were immunoprecipited by anti-5hmC antibody for Next-Generation Sequencing
|
| |
|
| Contributor(s) |
Sun J, Du J, Wu X, Li L, Wang J |
| Citation(s) |
30146412 |
| |
| Submission date |
Jul 19, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Sun Jie |
| E-mail(s) |
[email protected]
|
| Organization name |
City of Hope
|
| Lab |
Li Lab
|
| Street address |
1500 East Duarte Road
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE117383 |
SIRT1 Activation Disrupts Maintenance of Myelodysplastic Syndrome Stem and Progenitor Cells by Restoring TET2 Function |
|
| Relations |
| BioProject |
PRJNA481884 |
| SRA |
SRP154432 |
Less...
More...