|
| Status |
Public on Jun 27, 2019 |
| Title |
Genome-wide analysis of DNA methylation in KRAB-ZFP cluster KO ES cells |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
We report a retrotransposon-specific loss of CpG methylation in chromosome 4 KRAB-ZFP cluster (Chr4-cl) KO ES cells that are cultivated in conditions that induce DNA hypomethylation. The retrotransposon groups with the strongest DNA methylation loss are the main targets of the KRAB-ZFPs within the deleted gene cluster indicating that these KRAB-ZFPs prevent complete DNA demethylation at retrotransposons during global DNA demethylation.
|
| |
|
| Overall design |
Analysis of CpG methylation status in WT and Chr4-cl KO ES cells in serum and 2i + vitamin C growth condition,
|
| |
|
| Contributor(s) |
Wolf G, Macfarlan T, Sun M |
| Citation(s) |
32479262 |
| |
| Submission date |
Jun 04, 2018 |
| Last update date |
Jun 15, 2020 |
| Contact name |
Gernot Wolf |
| Organization name |
NIH
|
| Department |
NICHD
|
| Lab |
Macfarlan lab
|
| Street address |
6 Center Drive
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE115291 |
Retrotransposon reactivation and mobilization upon deletions of megabase-scale KRAB zinc finger gene clusters in mice |
|
| Relations |
| BioProject |
PRJNA474491 |
| SRA |
SRP149701 |
Less...
More...