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| Status |
Public on Jun 14, 2019 |
| Title |
Landscapes of gene translation in hepatocellular carcinoma tumors revealed by ribosome profiling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Despite the critical role of translation in the multi-level gene expression regulation program that controls the protein abundance, a high-resolution view on the landscapes of gene translation in cancer has been missing. We used the technique of ribosome profiling to dissect the translatomes of human liver tumors and their adjacent normal tissues. Our results, as a novel resource, revealed genome-wide abnormalities of translation efficiencies in tumors and context-dependent non-canonical ORFs.
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| Overall design |
We profiled the paired tumor and adjacent normal tissue samples from 10 patients of HCC by the procedure of ribosome profiling, which was adapted and optimized for processing of clinical solid tissue samples. Total RNA sequencing was performed in parallel for the same samples.
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| Contributor(s) |
Zou Q, Huang R, Xiao Z, Yang X |
| Citation(s) |
31281537 |
| |
| Submission date |
Apr 04, 2018 |
| Last update date |
Jul 16, 2019 |
| Contact name |
Xuerui Yang |
| E-mail(s) |
[email protected]
|
| Organization name |
Tsinghua University
|
| Department |
School of Life Sciences
|
| Street address |
Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA448763 |
| SRA |
SRP137150 |
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