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| Status |
Public on Apr 15, 2008 |
| Title |
Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus |
| Organism |
Caldicellulosiruptor saccharolyticus |
| Experiment type |
Expression profiling by array
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| Summary |
Caldicellulosiruptor saccharolyticus is an extremely thermophilic, Gram-positive anaerobe, which ferments cellulose-, hemicellulose- and pectin-containing biomass to acetate, CO2 and hydrogen. Its broad substrate range, high hydrogen-producing capacity, and ability to co-utilize glucose and xylose, make this bacterium an attractive candidate for microbial bioenergy production. Glycolytic pathways and an ABC-type sugar transporter were significantly up-regulated during growth on glucose and xylose, indicating that C. saccharolyticus co-ferments these sugars unimpeded by glucose-based catabolite repression. The capacity to simultaneously process and utilize a range of carbohydrates associated with biomass feedstocks represents a highly desirable feature of a lignocellulose-utilizing, biofuel-producing bacterium.
Keywords: substrate response
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| Overall design |
C. saccharolyticus was subcultured (overnight) 3 times on the substrate of interest in modified DSMZ 640 medium before inoculating a pH-controlled (pH = 7) 1-liter fermentor containing 4 gram substrate per liter. Cells were grown at 70 °C until mid-logarithmic phase (~OD660 = 0.3-0.4) and harvested by centrifugation and rapid cooling to 4 °C and stored at -80 °C. To elucidate the central carbon metabolic pathways and their regulation, transcriptome analysis was performed after growth on glucose, xylose and a 1:1 mixture of both substrates. L-Rhamnose, which was likely to follow another pathway, was used as a reference substrate.
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| Contributor(s) |
VanFossen AL, van de Werken HJ, Verhaart MR |
| Citation(s) |
18776029 |
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| Submission date |
Apr 11, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Amy VanFossen |
| Organization name |
NC State Unversity
|
| Department |
Chemical and Biomolecular Engineering
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| Lab |
Kelly Group
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| Street address |
840 Main Campus Dr. Rm3305
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| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27606 |
| Country |
USA |
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| Platforms (1) |
| GPL6681 |
NCSU_Caldicellulosiruptor saccharolyticus_3K array_version 1 |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA106957 |
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