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Platform GPL6681 Query DataSets for GPL6681
Status Public on Apr 09, 2008
Title NCSU_Caldicellulosiruptor saccharolyticus_3K array_version 1
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Caldicellulosiruptor saccharolyticus
Manufacturer NCSU Kelly group
Manufacture protocol A DNA microarray was designed and constructed based on 2793 protein-coding sequences in the C. saccharolyticus genome obtained from the Department on Energy's Joint Genomes Institute (http://genome.ornl.gov/microbial/csac). OligoArray 2.0 (Rouillard, 2003) was used to generate one 60-mer oligonucleotide probe sequence for each open reading frame. The probes were synthesized (Integrated DNA Technologies, IA), re-suspended in 50% DMSO, and printed onto Ultragap microarray slides (Corning, NY) using a QArrayMini arrayer (Genetix, UK). Each probe was spotted five times onto each array to fortify statistical analysis.
 
 
Contributor(s) VanFossen AL
Submission date Apr 03, 2008
Last update date Apr 09, 2008
Contact name Amy VanFossen
Organization name NC State Unversity
Department Chemical and Biomolecular Engineering
Lab Kelly Group
Street address 840 Main Campus Dr. Rm3305
City Raleigh
State/province NC
ZIP/Postal code 27606
Country USA
 
Samples (42) GSM280522, GSM280523, GSM280524, GSM280525, GSM434336, GSM434343 
Series (9)
GSE11153 Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus
GSE17425 Csac growth on monosaccharides found in lignocellulose
GSE17427 Csac growth on model substrates found in lignocellulose

Data table header descriptions
ID
ORF gene locus
PT_GI
GeneID
SEQUENCE

Data table
ID ORF PT_GI GeneID SEQUENCE
Csac_0001 Csac_0001 146295086 5086606 AATGTACTTAGCAAGAGAGCTCACAAATGTATCCTTGCCTAAAATAGGGGAAGCCTTTGG
Csac_0002 Csac_0002 146295087 5087120 ATGGAGGAGTAGACATTTACATTACATCACTTACAAGCCCGAGCGTTGTAAAGCAGCAAG
Csac_0003 Csac_0003 146295088 5089342 CAGTTTGAAAAGGATGTTCAGATGGGGTTTACAACCAAAGGTGTTCACAGAGATGATTTT
Csac_0004 Csac_0004 146295089 5087507 ATAATCCTTGAGATTGATGGAAGACTGTATGCTATTGTCTCTCCTGTGTCCTCAACAACA
Csac_0005 Csac_0005 146295090 5087508 TTTGAGAGTTGAACTTGAAGATGCTGTCATGGCAGAGGAGATTTTCACAATTTTGATGGG
Csac_0006 Csac_0006 146295091 5086612 CAAGTTCCTGTTCAGGGAAGAAATACACAGGGTGTTAAACTTATGCGAATTGATAGCGAT
Csac_0007 Csac_0007 146295092 5086827 ATATTGGGGATGTGCTCTACTCAGTTTCAAACTCAATGATAAAAGCAAGCTCTCTTGAAA
Csac_0008 Csac_0008 146295093 5088235 ACCAAGGCTTGTAAAGGAAGACAAAGAAAAGTACGACGAACTTGCACAAAGAGTTGAACA
Csac_0009 Csac_0009 146295094 5088131 TTGGTTACTGGTGGATAAAAGAATATGAACCGAACACTGCAACAATAGTGCCGGCTCATA
Csac_0011 Csac_0011 146295095 5087505 ATGTTGAGGGTGAAAAGAGATTGCCTGTTAGAACAAAAGAACCTATACCAAAAGAATTGC
Csac_0012 Csac_0012 146295096 5087218 GACTCAAGTAAGCTCAAACAAGAAATAATTGAGGAGTGGAAGGAGATTTTGAAGAACTAT
Csac_0013 Csac_0013 146295097 5087219 TTTAGGAAAGGACGTTGTTGTAATATCACCTGAGAGGATAAGGAATGAGATAATTTCAAG
Csac_0014 Csac_0014 146295098 5086611 CTTACTTTGGACTACTTGAGATGAAAACCACCAATGATGTTAGAAAAGGAAGAGAGAATG
Csac_0015 Csac_0015 146295099 5086925 GCAATTGATTTAGAAGTTCACAAAAGCACTCGCAATTATAAGGATTGGCAGAAAGAAGAA
Csac_0019 Csac_0019 146295100 5087986 TTGAAAGGTTCAAATTTGAAATATGACATAAAATCTCCTTCTTTTGTGATTATAGATTAG
Csac_0020 Csac_0020 146295101 5086685 CAGAGGGAATAGAGAACAATAAACAGCTTGAGATTTTAAAAGAGATTGGCTGTGATTTTG
Csac_0021 Csac_0021 146295102 5086682 TTTGCAGAATATAAAAGGGATAAAGATTCAGAATGTCTTTGTTACCAGCAGCGGAGAGAT
Csac_0022 Csac_0022 146295103 5087575 AATTGCTCGTGGTAAGGTTGCTCCAAACGGAATAGACCTTTATGTAATACCTGCATTTGC
Csac_0023 Csac_0023 146295104 5087154 ATGCAGCTTGTATATTTCCACAAACTGCTCAATATTCACGAAAGGTTCAAAGAAAAGTAG
Csac_0024 Csac_0024 146295105 5086585 AGGACATTAGCAAAAGAAGTTTTAGAATACCTTCCGCTAAGAGATATGCTGGAGAAAGAT

Total number of rows: 2679

Table truncated, full table size 258 Kbytes.




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