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| Status |
Public on Feb 16, 2018 |
| Title |
Translatome of developing drosophila t4/t5 neurons in wildtype versus atonal loss of function genotypes |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Atonal is a proneural transcription factor expressed in the Drosophila neuroblast cluster known as the inner proliferation center (IPC). The t4/t5 neurons that derived from the IPC require atonal for their normal development. To characterize the role of Atonal in t4/t5 development we have sequenced the translated RNA in t4/t5 neurons in wild type and ato loss of function flies.
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| Overall design |
To sequence the translated RNA in wild type and ato loss of function t4/t5 neurons we have collected brains from transgenic larvae that in t4/t5 neurons express the ribosomal protein Rbp10Ab tagged with GFP. A 10% of the brain homogenates was used as input, the rest was immunuprecipated with anti-GFP antibodies to isolate t4/t5 ribosomes. The RNA bound to the ribosomes was later purified sonicated and sequenced.
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| Contributor(s) |
Mora N, Fiers M, Hassan B |
| Citation(s) |
29576424 |
| |
| Submission date |
Feb 15, 2018 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Mark Fiers |
| E-mail(s) |
[email protected]
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| Organization name |
VIB
|
| Department |
Center for Brain and Disease
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| Street address |
Herestraat 49
|
| City |
Leuven |
| ZIP/Postal code |
3000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA434323 |
| SRA |
SRP132957 |
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