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Series GSE1089

Query DataSets for GSE1089

Status

Public on Mar 19, 2008

Title

Normal Calorie Restricted

Organism

Mus musculus

Experiment type

Expression profiling by array

Summary

Normal female Ames mouse (+/+ or +/df) with ad libitum access to water and fed 30% less of the standard pelleted diet with respect to the amount consumed by normal female ad libitum fed +/+ and +/df mice (LabDiet, PMI Feeds, Inc., St Louis, MO) caged with microisolator filter tops. Mice were killed between 24 to 26 months of age, tissues removed, rapidly frozen on dry ice, and stored in liquid nitrogen. Total liver RNA was isolated from frozen tissue as described (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. Bartke, S.R. Spindler, Physiological Genomics, Submitted). mRNA levels were measured using the Affymetrix mouse U74Av2 array according to standard protocols. After hybridization, arrays were scanned using a Hewlett-Packard GeneArray Scanner. Image analysis was performed as described (Cao SX, Dhahbi JM, Mote PL, and Spindler SR. Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice. Proc Natl Acad Sci U S A 98: 10630-10635, 2001). A more detailed description of the methods can be found in (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. Bartke, S.R. Spindler, Physiological Genomics, Submitted).
Keywords = Ames dwarf
Keywords = mouse
Keywords = Affymetrix
Keywords = caloric restriction
Keywords: parallel sample

Contributor(s)

Tsuchiya T, Dhahbi JM, Cui X, Mote PL, Bartke A, Spindler SR

Citation(s)

15039484

Submission date

Mar 02, 2004

Last update date

Feb 18, 2018

Contact name

Stephen R. Spindler

E-mail(s)

[email protected]