Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing
Summary
Comparative RNA and small RNA sequencing analysis of in vitro differentiated β like cells, in vivo matured β cells, adult islet cells, and Lin28 deficient in vitro differentiated β like cells
Overall design
We performed RNA-seq and small-RNA seq on human ESCs differentiated into β like cells in vitro, same cells transplanted into kidney capsule of mouse recipients, and fresh human islets. We also performed RNA-seq on hESCs genomically modified with dox inducible CRISPR knockout of LIN28 and then differentiated into β like cells plus/mius doxycycline
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