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| Status |
Public on Oct 11, 2018 |
| Title |
Time-course transcriptome of regeneration [RNA-Seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The ability to regenerate or recover from injuries varies greatly not only between species but also between tissues and organs or developmental stages of the same species. The mechanisms behind these different regenerative capabilities are ultimately dependent on the control of genome activity, determined by a complex interplay of regulatory elements functioning at the level of chromatin. Resetting of gene expression patterns during injury responses is, thus, shaped by the coordinated action of genomic regions (enhancers, silencers) that integrate the activity of multiple sequence-specific DNA binding proteins (transcription factors and cofactors). Using genome- wide approaches to interrogate chromatin function here we identify the regulatory elements governing tissue recovery in Drosophila wing imaginal discs, which show a high regenerative capacity after genetically induced cell death. Our findings point to a global co-regulation of gene expression and provide evidence for Damage Responding Regulatory Elements (DRRE), some of which are novel whereas others are also used in other tissues or developmental stages.
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| Overall design |
We collected data at different time points (0, 15 and 25h) after apoptosis induction. These time periods were selected because they included the most important transcriptional responses to apoptosis, ranging from the earliest gene expression up to complete re-patterning. Discs kept at the same conditions without inducing cell death were used as controls.
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| Contributor(s) |
Vizcaya E, Klein CC, Serras F, Mishra R, Guigó R, Corominas M |
| Citation(s) |
30459214, 39157585 |
| |
| Submission date |
Aug 19, 2017 |
| Last update date |
Aug 21, 2024 |
| Contact name |
Montserrat Corominas |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Barcelona
|
| Department |
Genètica, Microbiologia i estadística
|
| Street address |
Diagonal 643
|
| City |
Barcelona |
| State/province |
Catalonia |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE102841 |
Time-course chromatin accessibility and transcriptome in regeneration |
|
| Relations |
| BioProject |
PRJNA399614 |
| SRA |
SRP116025 |
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