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| Status |
Public on Jan 16, 2008 |
| Title |
Comparison of gene expression in the epidermis of Tcfap2c mutant and control skin at embryonic day 16.5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The development of the epidermis, a stratified squamous epithelium, is dependent on the regulated differentiation of keratinocytes. Differentiation begins with the initiation of stratification, a process tightly controlled through proper gene expression. AP-2γ is expressed in skin and previous research suggested a pathway where p63 gene induction results in increased expression of AP-2γ which in turn is responsible for induction of K14. This study uses a conditional gene ablation model to further explore the role of AP-2γ in skin development. Mice deficient for AP-2γ exhibited delayed expression of p63, K14, and K1, key genes required for development and differentiation of the epidermis. In addition, microarray analysis of E16.5 skin revealed delayed expression of additional late epidermal differentiation genes: filaggrin, repetin and secreted Ly6/Plaur domain containing 1, in mutant mice. The genetic delay in skin development was further confirmed by a functional delay in the formation of an epidermal barrier. These results document an important role for AP-2γ in skin development, and reveal the existence of regulatory factors that can compensate for AP-2γ in its absence.
Keywords: genetic modification
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| Overall design |
Skin samples were collected from 3 Tcfap2c and 3 control animals at embryonic day 16.5.
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| Contributor(s) |
Winger QA, Guttormsen J, Koster MI, Stevens JR, Roop DR, Williams T |
| Citation(s) |
18353300 |
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| Submission date |
Jan 14, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Jill Guttormsen |
| E-mail(s) |
[email protected]
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| Phone |
435-797-5486
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| Organization name |
Utah State University
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| Department |
ADVS
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| Lab |
Winger Lab
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| Street address |
5600 Old Main Hill
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| City |
Logan |
| State/province |
UT |
| ZIP/Postal code |
84322 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA108383 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE10175_RAW.tar |
24.0 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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