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| Status |
Public on Jul 12, 2018 |
| Title |
Dynamic placement of the linker histone H1 regulates nucleosome arrangement and coordinates gene transcription in early Drosophila embryos[ChIP-Seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
H1 ChIP-seq profiling and nucleosome MNase-seq profiling were performed in after egg laying (AEL) 3-4 hours (hr) and 14-15hr Drosophila melanogaster embryos (W1118).
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| Overall design |
Nucleosome and H1 maps were generated from AEL 3-4hr and 14-15hr D. melanogaster embryos. We determinated nucleosome occupancy in embryo by micrococcal nuclease sequencing (MNase-Seq). We also performed ChIP-Seq (chromatin immunoprecipitation and sequencing) for H1 genome wide profiles. Sequencing was performed on Illumina HiSeq 2000 using signle end protocol.
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| Contributor(s) |
Hu J, Jiang C |
| Citation(s) |
29988149 |
| |
| Submission date |
Jul 12, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jian Hu |
| E-mail(s) |
[email protected]
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| Organization name |
Tongji University
|
| Street address |
1239,Siping Road
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE101330 |
Dynamic placement of the linker histone H1 regulates nucleosome arrangement and coordinates gene transcription in early Drosophila embryos |
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| Relations |
| BioProject |
PRJNA394011 |
| SRA |
SRP111731 |
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