GEO DataSets

Analysis of the axonal compartment of cultured motoneurons depleted for survival motor neuron (Smn). Smn is the protein deficient in spinal muscular atrophy (SMA). Results provide insight into the axonal mRNA content of degenerating motoneurons in vitro.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 genotype/variation sets

Platform:

GPL1261

Series:

GSE59506

9 Samples

Download data: CEL

(Submitter supplied) Neuronal function critically depends on coordinated subcellular distribution of mRNAs. Disturbed mRNA processing and axonal transport has been found in spinal muscular atrophy and could be causative for dysfunction and degeneration of motoneurons. Despite the advances made in characterizing the transport mechanisms of several axonal mRNAs, an unbiased approach to identify the axonal repertoire of mRNAs in healthy and degenerating motoneurons has been lacking. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS5459 GDS5460

Platform:

GPL1261

18 Samples

Download data: CEL

Analysis of the somatodendritic compartment of cultured motoneurons depleted for survival motor neuron (Smn). Smn is the protein deficient in spinal muscular atrophy (SMA). Results provide insight into the somatodendritic mRNA content of degenerating motoneurons in vitro.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 genotype/variation sets

Platform:

GPL1261

Series:

GSE59506

9 Samples

Download data: CEL

(Submitter supplied) Transcriptiome profiling of SMN MO injected, Gemin2 MO injected zebrafish embryos vs Control MO injected

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL10182

13 Samples

Download data: GPR

(Submitter supplied) Spinal muscular atrophy (SMA) is the most common genetic cause of infant mortality, with an incidence of around 1 in 6,000-10,000 live births. SMA is caused by low levels of full-length survival of motor neuron protein (SMN). We demonstrate that SMN binds to ribosomes and that this interaction is tissue-dependent. We performed ribosome profiling analyses on lysates from P5 wild-type mouse brains exposed to RNase I, from which we isolated SMN-primed ribosomes by immunoprecipitation. more...

Organism:

Mus musculus

Type:

Other

Platform:

GPL17021

8 Samples

Download data: TXT

(Submitter supplied) Several studies indicate that SMN-containing mRNP complexes could be involved in the axonal localization of a large number of mRNAs. We have used murine motor neuron-like NSC-34 cells and RNA Immuno-Precipitation experiments coupled to microarray analyses to perform a genome-wide analysis of RNA species present in mRNP complexes containing the full length SMN protein (flSMN). In situ hybridization and immuno-fluorescence experiments performed on several candidates indicate that these mRNAs colocalize with the SMN protein in neurites and axons of differentiated NSC-34 cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

5 Samples

Download data: CEL

(Submitter supplied) Protein inclusions containing the RNA-binding protein TDP-43 are a pathological hallmark of amyotrophic lateral sclerosis and other neurodegenerative disorders. The loss of TDP-43 function that is associated with these inclusions affects post-transcriptional processing of RNAs in multiple ways including pre-mRNA splicing, nucleocytoplasmic transport, modulation of mRNA stability and translation. In contrast, less is known about the role of TDP-43 in axonal RNA metabolism in motoneurons. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16417

20 Samples

Download data: CSV

(Submitter supplied) Spinal muscular atrophy (SMA) is the most common genetic cause of infant mortality, with an incidence of around 1 in 6,000-10,000 live births. SMA is caused by low levels of full-length survival of motor neuron protein (SMN). Approximately 95% of SMA cases in humans are caused by homozygous deletion of SMN1, with a smaller number caused by discrete mutations within the gene. In concert with other RNA binding proteins, SMN forms complexes both in the nucleus and the cytoplasm of neurons. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

20 Samples

Download data: TXT

(Submitter supplied) Spinal muscular atrophy with respiratory distress type 1 (SMARD1) is a fatal motoneuron disorder in children with unknown etiology. The disease is caused by mutations in the IGHMBP2 gene, encoding a Super Family 1 (SF1)-type RNA/DNA helicase. IGHMBP2 is a cytosolic protein that binds to ribosomes and polysomes, suggesting a role in mRNA metabolism. Here we performed morphological and functional analyses of isolated Ighmbp2-deficient motoneurons to address the question whether the SMARD1 phenotype results from de-regulation of protein biosynthesis. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

6 Samples

Download data: CSV

(Submitter supplied) Disturbed RNA processing and subcellular transport contribute to the pathomechanisms of motoneuron diseases such as amyotrophic lateral sclerosis and spinal muscular atrophy. RNA-binding proteins are involved in these processes, but the mechanisms how they regulate the subcellular diversity of transcriptomes, in particular in axons, are not understood. hnRNP R interacts with several proteins involved in motoneuron diseases. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL11002 GPL16417

33 Samples

Download data: TXT

(Submitter supplied) Local protein synthesis in sensory neuron axons is necessary for axonal regeneration with the efficiency of regeneration decreasing with age. Because the full repertoire of transcripts in embryonic and adult rat sensory axons is unknown we asked how the pool of mRNAs dynamically changes during ageing. We isolated mRNA from pure axons and growth cones devoid of non-neuronal or cell body contamination. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

10 Samples

Download data: CEL

(Submitter supplied) To analyze axon transcriptome of dentate gyrus granule cells, dorsal root ganglion cells and retinal ganglion cells, we perfomed axon RNA sequencing.Neurons were cultured in microfluidic chambers and axons were isolated. Total RNA of axons was collected separately and subjected to library construction using M01440v2 NuGEN Trio RNA-seq kit. After sequencencing,we used TPM method to normalize RNA-seq reads.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23479

3 Samples

Download data: TXT