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| Status |
Public on Nov 29, 2013 |
| Title |
Genome wide analysis of RNA species present in SMN-containing RNPs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Several studies indicate that SMN-containing mRNP complexes could be involved in the axonal localization of a large number of mRNAs. We have used murine motor neuron-like NSC-34 cells and RNA Immuno-Precipitation experiments coupled to microarray analyses to perform a genome-wide analysis of RNA species present in mRNP complexes containing the full length SMN protein (flSMN). In situ hybridization and immuno-fluorescence experiments performed on several candidates indicate that these mRNAs colocalize with the SMN protein in neurites and axons of differentiated NSC-34 cells. Moreover, they localize in cell processes in a SMN-dependent manner. Thus, low SMN levels might result in localization deficiencies of mRNAs required for axonogenesis.
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| Overall design |
RNA species coimmunoprecipitated with the SMN protein were used for hybridization of Affymetrix microarrays
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| Contributor(s) |
Rage F, Boulisfane N, Neel H, Gostan T, Bertrand E, Bordonné R, Soret J |
| Citation(s) |
24152552 |
| |
| Submission date |
Aug 17, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
johann Soret |
| E-mail(s) |
[email protected]
|
| Organization name |
CNRS
|
| Department |
Institut de Genetique Moleculaire
|
| Lab |
Assembly and traffic of ribonucleoproteins
|
| Street address |
1919, route de Mende
|
| City |
Montpellier |
| ZIP/Postal code |
34293 |
| Country |
France |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA173074 |
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