GEO DataSets

Analysis of FACS-sorted dermal macrophage, epidermal Langerhans and CD14+ dendritic cell (DC) subsets. DCs, monocytes and macrophages are leukocytes with critical roles in immunity. Result provide insight into molecular profiles distinguishing dermal DCs from monocyte-derived cells and macrophages.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 cell type, 6 individual sets

Platform:

GPL6884

Series:

GSE60317

11 Samples

Download data

(Submitter supplied) DC, monocyte and macrophage networks are evolutionarily conserved but the distinct subsets have been difficult to distinguish due to shared overlapping phenotypic markers between the cells. Using transcriptome microarray profiling of human and mouse mononuclear phagocyte subsets, we have distinguished human dermal DCs from monocyte-derived cells and macrophages.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5349

Platform:

GPL6884

11 Samples

Download data: TXT

(Submitter supplied) Numerous CD11b+ myeloid cells are present within the dermis. They are very heterogeneous and can be divided in dermal DCs, tissue monocytes and tissue macrophages. At steady state, only CD11b+ DC migrate from the dermis to the skin draining lymph nodes whereas upon DNFB-induced inflammation, CD11b+ DC as well as dermal monocytes migrated to the lymph nodes. The objective of this study was to use gene expression profiling to rigorously identify the different subsets of dermal CD11b+ myeloid cells at steady state and upon inflammation and to characterize their functional potential.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

38 Samples

Download data: CEL

(Submitter supplied) Dendritic cell (DC) are critical initiators and regulators of immunity to pathogens, vaccines, tumors and tolerance to self. Mouse and human dendritic cells (DCs) are comprised of functionally specialized subsets, but precise interspecies correlation is currently incomplete and hampers the full translation of murine findings to human DC-based clinical therapies. In this study, we show that murine lung and gut lamina propria CD11b+ DC populations are comprised of two subsets: FLT3- and IRF4-dependent CD24+CD64- DCs and contaminating CSF-1R-dependent CD24-CD64+ macrophages. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

6 Samples

Download data: TXT

(Submitter supplied) Dendritic cells (DCs) and macrophages (MPs) are important for immunological homeostasis in the colon. We found that F4/80hi CX3CR1hi (CD11b+CD103-) cells account for 80% of mouse colonic lamina propria (cLP) MHC-IIhi cells. Both CD11c+ and CD11c- cells within this population were identified as MPs based on multiple criteria, including a MP transcriptome revealed by microarray analysis. These MPs constitutively released high levels of IL-10 at least partially in response to the microbiota via an MyD88-independent mechanism. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4369

Platform:

GPL6246

10 Samples

Download data: CEL, CHP

Analysis of sorted, steady-state colonic cells subdivided into 4 populations: 2 DC subsets and 2 MP subsets based on CD11c and F4/80 expression. DCs and MPs are important for immunological homeostasis in colon. Results provide insight into molecular characterization of colonic MP and DC populations.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 4 cell type sets

Platform:

GPL6246

Series:

GSE27859

10 Samples

Download data: CEL, CHP

(Submitter supplied) The lineage relationships and fate of human blood and tissue dendritic cells (DC) has significance for a number of diseases including HIV where both blood and tissue DC may be infected. We used gene expression profiling of monocyte and DC sub-populations sorted directly from blood and skin and compared this to monocyte derived DC (MDDC) and MUTZ3 Langerhans cells (LCs) to define the lineage relationships. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL14674

62 Samples

Download data: GPR

(Submitter supplied) The lineage relationships and fate of human blood and tissue dendritic cells (DC) has significance for a number of diseases including HIV where both blood and tissue DC may be infected. We used gene expression profiling of monocyte and DC sub-populations sorted directly from blood and skin and compared this to monocyte derived DC (MDDC) and MUTZ3 Langerhans cells (LCs) to define the lineage relationships. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6884

24 Samples

Download data: TXT

(Submitter supplied) We use bulk RNA sequencing of sorted cells to characterize the gene expression profiles of renal MHCII+/-D11b+F4/80hi cells 72 hours after cisplatin-induced acute kidney injury (AKI). F4/80hi cells are activated after cisplatin induced AKI and start to downregulate MHCII on transcriptional level. Downregulation of MHCII is probably caused by a downregulation/inhibition of its most important transcriptional activator Ciita

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18480

8 Samples

Download data: CSV, TAB, TXT

(Submitter supplied) We use bulk RNA sequencing of sorted cells to characterize the gene expression profiles of renal dendritic cell (DC) subsets, cDC1 and cDC2, as well as MHCII+CD64+ F4/80hi and MHCII+CD64+ CD11bhi cells. Splenic DCs and red pulp macrophages serve as reference populations for a macrophage-like or DC-like phenotype. By sorting YFP+/YFP- F4/80hi or YFP+/YFP- CD11bhi cells from Clec9a-Cre Rosa-YFP mice we aim to reveal transcriptional differences between YFP-labelled and unlabelled cells. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18480

33 Samples

Download data: CSV, TAB, TXT

(Submitter supplied) Pulmonary dendritic cells are heterogenous cells comprise four distinct subsets including two conventional dendritic cell subsets, CD103+ and CD11bhiCD14lo cells, and two monocyte-derived dendritic cell subsets. Their functions in terms of migration and T cell activation are distinct, but genes regulating their features are to be determined. We used microarrays to identify a select set of genes that are expressed in conventinal dendritic cells and in monocyte-derived dendriti cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5663

Platform:

GPL1261

12 Samples

Download data: CEL, CHP

Analysis of four dendritic cell (DC) subsets purified from the lungs of C57BL/6 males following inhalation of LPS/OVA. The four subsets represent conventional DCs (cDCs) and monocyte-derived DCs (moDCs). Results provide insight into molecular profiles that would discriminate between cDCs and moDCs.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 cell type, 2 other sets

Platform:

GPL1261

Series:

GSE64896

12 Samples

Download data: CEL, CHP

(Submitter supplied) In comparison to murine dendritic cells (DCs), less is known about the function of human DCs in tissues. Here, we analyzed, using lung tissues from humans and humanized mice, the role of human CD1c+ and CD141+ DCs in determining the type of CD8+ T cell immunity to live-attenuated influenza virus (LAIV) vaccine. We found that both lung DC subsets acquired influenza antigens in vivo and expanded specific cytotoxic CD8+ T cells in vitro. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

18 Samples

Download data: TXT

(Submitter supplied) Transcriptomic profiling of ovine skin lymph dendritic cell subsets CD26+ and CD26- compared to total enriched lymph dendritic cells. CD26+ and CD26- dendritic cells (DC) from sheep skin lymph were sorted by flow cytometry and were analysed for their transcriptomic profile. We demonstrate that the minor sheep CD26+ skin lymph DC subset shares significant transcriptomic similarities with mouse CD8a+ and human BDCA3+ DC. more...

Organism:

Ovis aries

Type:

Expression profiling by array

Platform:

GPL10427

5 Samples

Download data: TXT

(Submitter supplied) Monocyte maturation program into macrophages (MΦ) is well-defined in murine gut under homeostatic or inflammatory conditions. Obviously, in vivo tracking of monocytes in inflamed tissues remains difficult in humans. Furthermore, in vitro models fall short in generating the surrogates of transient extravasated tissue inflammatory monocytes. Here, we aimed to unravel environmental cues that replicate in vitro the human monocyte “waterfall” process by first generating tissue-like inflammatory monocytes, and then shifted them towards MΦ. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23126

9 Samples

Download data: CEL

(Submitter supplied) Dendritic cells (DC) are mononuclear phagocytes which exhibit a dendritic morphology and excel at naïve T cell activation. DC encompass several subsets initially identified by their expression of specific cell surface molecules and later shown to possess distinct functions. DC subset differentiation is guided by different transcription factors and cytokines. Identifying DC subsets is challenging as very few cell surface molecules are uniquely expressed on any one of these cell populations and conventional flow cytometry analysis using limited antigens is biased and potentially misleading. more...

Organism:

Homo sapiens; Mus musculus

Type:

Third-party reanalysis; Expression profiling by array

Download data: TXT

(Submitter supplied) Plasmacytoid dendritic cells wre isolated from cutaneous lymph nodes of control C57BL/6 mice and used for microarray analysis.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

3 Samples

Download data: CEL

(Submitter supplied) We performed single-cell RNA-seq on CD14+ monocytes isolated from the blood of healthy donors. Using the 10x chromium technology, we analyzed 425 and 431 cells from 2 individual donors.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

2 Samples

Download data: CSV

(Submitter supplied) We analyzed the transcriptomes of human dendritic cells and macrophages derived from monocytes using MCSF + IL-4 + TNFa, or IL-34 + IL-4 + TNFa, or dendritic cells derived from monocytes using GMCSF + IL-4.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL11532

30 Samples

Download data: CEL

(Submitter supplied) Gene-expression microarray datasets generated as part of the Immunological Genome Project (ImmGen). Primary cells from multiple immune lineages are isolated ex-vivo, primarily from young adult B6 male mice, and double-sorted to >99% purity. RNA is extracted from cells in a centralized manner, amplified and hybridized to Affymetrix 1.0 ST MuGene arrays. Protocols are rigorously standardized for all sorting and RNA preparation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

653 Samples

Download data: CEL