GEO DataSets

Analysis of CD4 T-cells from 3-week old NOD females in the preinsulitis stage of Type 1 diabetes. Control strains NOR (~88% similarity to NOD genome) and C57BL/6 (genetically distant) are both insulitis- and diabetes-free. Results provide insight into molecular basis of CD4 T-cell diabetogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 disease state, 3 strain sets

Platform:

GPL1261

Series:

GSE46600

15 Samples

Download data: CEL

(Submitter supplied) Type 1 diabetes is a multigenic disease caused by T-cell mediated destruction of the insulin producing β-cells. Although conventional (targeted) approaches of identifying causative genes have advanced our knowledge of this disease, many questions remain unanswered. Using a whole molecular systems study, we unraveled the genes/molecular pathways that are altered in CD4 T-cells from young NOD mice prior to insulitis (lymphocytic infiltration into the pancreas). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS5018 GDS5019 GDS5020

Platform:

GPL1261

44 Samples

Download data: CEL

Analysis of CD4 T-cells from 4-week old NOD females in the preinsulitis stage of Type 1 diabetes. Control strains NOR (~88% similarity to NOD genome) and C57BL/6 (genetically distant) are both insulitis- and diabetes-free. Results provide insight into molecular basis of CD4 T-cell diabetogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 disease state, 3 strain sets

Platform:

GPL1261

Series:

GSE46600

15 Samples

Download data: CEL

Analysis of CD4 T-cells from 2-week old NOD females in the preinsulitis stage of Type 1 diabetes. Control strains NOR (~88% similarity to NOD genome) and C57BL/6 (genetically distant) are both insulitis- and diabetes-free. Results provide insight into molecular basis of CD4 T-cell diabetogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 disease state, 3 strain sets

Platform:

GPL1261

Series:

GSE46600

14 Samples

Download data: CEL

(Submitter supplied) Analysis of gene expression levels in ex-vivo and p79-stimulated splenic CD4+ T cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL14828

12 Samples

Download data: TXT

(Submitter supplied) Islet leukocytic infiltration (insulitis) is first obvious at around 4 weeks of age in the NOD mouse – a model for human type 1 diabetes (T1DM). The molecular events leading to insulitis are poorly understood. Since TIDM is caused by numerous genes, we hypothesized that multiple molecular pathways are altered and interact to initiate this disease. Analysis of the global gene expression profiles using microarrays followed by hierarchical clustering revealed that the majority (~90%) of the differentially expressed genes in NOD mice relative to control mice were repressed. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL339 GPL340

60 Samples

Download data: CEL

(Submitter supplied) Type 1 Diabetes (T1D) in humans and the non-obese diabetic (NOD) mouse model results from autoreactive T cell destruction of pancreatic beta cells. A pathogenic role for B lymphocytes (B cells) in T1D first became evident when NOD mice made deficient in this population through introduction of an inactivated Igµ heavy chain gene (NOD.Igµnull) or chronic treatment with anti-IgM antibodies were strongly protected from disease. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4340

Platform:

GPL1261

11 Samples

Download data: CEL

Analysis of anti-IgM-F(ab’)2 fragment-stimulated, splenic B cells from non-obese diabetic (NOD) or NR4 (NOD background with Chromosome 4, type 1 diabetes (T1D)-resistance alleles) females. Results provide insight into the molecular mechanisms underlying NOD and NR4 diabetogenic activity.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 2 strain sets

Platform:

GPL1261

Series:

GSE37294

11 Samples

Download data: CEL

(Submitter supplied) The aim of this study is to identify genes implicated in the early steps of the autoimmune process, prior to inflammation in type 1 diabetes. Early Insulin AutoAntibodies (E-IAA) have been used as subphenotypic marker to select individual animals as type 1 diabetes prone and to compare gene expression patterns with insulin autoantibody negative NOD. Variation of gene expression patterns in the pancreatic lymph nodes (PLN) issued from E-IAA positive and negative mice have been analyzed by DNA microarrays

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4024

Platform:

GPL81

9 Samples

Download data: CEL

Analysis of pancreatic lymph nodes of Early Insulin AutoAntibodies (E-IAA) positive 5 wk-old Non Obese Diabetic (NOD) mice. E-IAA mark the first measurable phenotypic checkpoint in T1D pathogenesis. Results provide insight into molecular mechanisms underlying initiation of T1D.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL81

Series:

GSE15582

9 Samples

Download data: CEL

(Submitter supplied) Both immunodeficient and wild type NOD mice exhibit defects in control of early T-cell development in the thymus. We show that Rag1-deficient NOD mice fail to enforce both the b-selection checkpoint and an earlier T-cell commitment checkpoint, based on genome-wide genetic and transcriptome analyses. A major QTL peak for the checkpoint breakthrough phenotype mapped to the diabetes susceptibility Idd9/11 region, as confirmed by congenic mouse analysis. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9250

4 Samples

Download data: RPKM

(Submitter supplied) Cytosolic and mitochondrial fragments that arise from the cleavage of tRNAs and mt-tRNAs, respectively, have been identified as potential novel regulators of cellular functions. In this study we identified hundreds of fragments in the pancreatic islets of healthy human individuals. We were able to annotate 3593 tRFs (16-55 base pairs) present in the islets of healthy human individuals (we considered a minimum of 5 cpm values in each of the 3 samples). more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16791

3 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11154

48 Samples

Download data

(Submitter supplied) ChIPseq analysis of survivin chromatin binding in activated CD4+ T cells

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11154

8 Samples

Download data: XLSX

(Submitter supplied) CD4 T cells from 4 healthy women were activated with aCD3 and treated with the survivin inhibitor YM155, 10 ng/ml or control during 24 hours. The last 2 hours with upplement of IFNg. The objective was to understand the action of survivin (coded by BIRC5 gene) in CD4 T cells.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

8 Samples

Download data: XLSX

(Submitter supplied) CD4 T cells from 4 healthy women were activated with aCD3 and treated with the survivin inhibitor YM155, 10 ng/ml or control during 72 hours. The last 2 hours with upplement of IFNg. The objective was to understand the action of survivin (coded by BIRC5 gene) in CD4 T cells.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

8 Samples

Download data: XLSX

(Submitter supplied) RNAseq of CD4 cells from 24 female RA patients activated with aCD3 for 48 hours. Patients were collected crossectionally from the Rheumatology clinic during 2018. Age md 47, range 24-67 years, Disease duration md 5.5, range 1-30 years.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

24 Samples

Download data: XLSX