GEO DataSets

Analysis of anterior and posterior brain regions from Retinaldehyde Dehydrogenase 2 null-mutant (Raldh2-/-) embryos, which are unable to synthesize retinoic acid (RA) from maternally-derived retinol. Results provide insight into the repertoire of RA-regulated genes in the developing embryo.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 development stage, 2 genotype/variation, 2 tissue sets

Platform:

GPL6246

Series:

GSE43578

18 Samples

Download data: CEL, CHP

(Submitter supplied) Retinoic acid (RA), an active derivative of the liposoluble vitamin A (retinol), acts as an important signaling molecule during embryonic development, regulating phenomenons as diverse as anterior-posterior axial patterning, forebrain and optic vesicle development, specification of hindbrain rhombomeres, pharyngeal arches and second heart field, somitogenesis, and differentiation of spinal cord neurons. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4836

Platform:

GPL6246

18 Samples

Download data: CEL, CHP

(Submitter supplied) Retinoic acid (RA) is an important developmental signaling molecule responsible for the patterning of multiple vertebrate tissues. RA is also a potent teratogen, causing multi-organ birth defects in humans. Endogenous RA levels must therefore be tightly controlled in the developing embryo. In order to understand the RA function and regulation at the genomic level, we used a microarray approach to identify genes that function as negative feedback regulators of retinoic acid signaling. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL1319

16 Samples

Download data: CEL

(Submitter supplied) Vertebrate axial skeletal patterning is controlled by coordinated collinear expression of Hox genes and axial level-dependent activity of Hox protein combinations. Transcription factors of the Meis family act as cofactors of Hox proteins and profusely bind to Hox complex DNA, however their roles in mammalian axial patterning have not been established. Similarly, retinoic acid (RA) is known to regulate axial skeletal element identity through the transcriptional activity of its receptors, however whether this role is related to Meis/Hox regulation or functions in axial patterning remains unknown. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

18 Samples

Download data: TXT

(Submitter supplied) Retinoic acid (RA), the bioactive derivative of vitamin A, is essential for vertebrate heart development. Both excess and reduced RA signaling lead to cardiovascular malformations, particularly affecting the cardiac outflow tract (OFT). The cellular mechanisms underlying the effects of RA signaling during OFT morphogenesis are not fully understood. To address this question, we used transient maternal RA supplementation to rescue the early lethality resulting from inactivation of the murine retinaldehyde dehydrogenase 2 (Raldh2) gene. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13912

8 Samples

Download data: TXT

(Submitter supplied) Retinoid signaling is important for patterning the vertebrate hindbrain and midaxial regions. We recently showed that signaling through retinoic acid receptors (RARs) is essential for anteroposterior patterning along the entire body axis. To further investigate the mechanisms through which RARs act, we employed microarray analysis to investigate the effects of modulating RAR activity on target gene expression. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL1860

3 Samples

Download data

(Submitter supplied) This series represents murine dorsal neural tube bisected along the midline with one half from each embryo used for control and the other half treated with 10-6M RA dissolved in ethanol for 6, 12, 24 or 48 h. For 6 h exposures, the explants were cultured overnight on fibronectin coated 35mm dishes (Biocoat, Becton Dickinson Labware, Bedford, MA) in DMEM with 10% horse serum in order to allow for sufficient outgrowth of neural crest cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS773

Platform:

GPL81

27 Samples

Download data: CEL, EXP

Expression profiling of cranial neural crest exposed to 1 uM retinoic acid (RA) at various lengths of time up to 48 hours. Neural crest obtained from 8.5 days postcoitum ICR embryos. Results provide insight into developmental pathways affected upon exposure to teratogenic concentrations of RA.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 4 time sets

Platform:

GPL81

Series:

GSE1588

27 Samples

Download data: CEL, EXP

(Submitter supplied) Wild type C57Bl/6J, Cyp1b1-null, and a substrain of Cyp1b1-null that are resistant to diet-induced obesity (Resistant Cyp1b1-null) timed mated pregnant dams were administered either a defined vitamin A sufficient diet or matched vitamin A deficient diet from embryonic day 4.5. Offspring liver gene expression was examined at birth (post-natal day 0) and at weaning (post-natal day 21).

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

24 Samples

Download data: TXT

(Submitter supplied) To better understand the role of Hoxa1 during embryogenesis and gain insight to the transcriptional network controlled by this gene, we carried out a large-scale screening for Hoxa1 downstream targets by performing microarray analysis. Tissue from the rhombomere 3-5 region of wildtype and Hoxa1 null embryos, including neuroectoderm, mesoderm and otic ectoderm was microdissected at the peak of Hoxa1 expression. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5228

Platform:

GPL7202

8 Samples

Download data: TXT

Analysis of rhombomere regions 3 to 5 from embryos lacking the homeobox protein Hoxa1. Hoxa1 is necessary for the development of the brainstem, inner ear and heart. Results identify the role of Hoxa1 during embryogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL7202

Series:

GSE25868

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other

Platforms:

GPL21741 GPL24776

62 Samples

Download data: BEDGRAPH, BW, NARROWPEAK, TXT

(Submitter supplied) Retinoid homeostasis is critical for normal embryonic development, and both the deficiency and excess of these compounds are associated with congenital malformations. Here we found that SIRT1, the most conserved mammalian NAD+-dependent deacetylase, contributes to the maintenance of homeostatic retinoic acid (RA) signaling and modulates mouse embryonic stem cell (mESC) differentiation. Our data show that SIRT1 deficiency enhances RA signaling, thereby accelerating mES cell differentiation in response to RA. more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

Platform:

GPL4133

16 Samples

Download data: TXT

(Submitter supplied) Aims: Congenital heart defect (CHD) account for 25% of all human congenital abnormalities. Very few CHD-causing genes have been identified so far; so, to discover further genes we performed a global transcriptome analysis in mouse models of CHD. Methods and results: By the use of a retinoic acid competitive antagonist (BMS-189453) we caused CHD, thymic abnormalities and neural tube defects in mouse newborns. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL9669

2 Samples

Download data: GPR

(Submitter supplied) Retinoic acid (RA) is known to regulate many genes during development by acting as a ligand for nuclear RA receptors that bind RA response elements (RAREs). However, identification of RAREs required to activate or repress specific genes during development has been quite difficult. Here, we focused on identification of RAREs in the developing trunk of vertebrate embryos during the early stages of body axis extension when RA controls neuromesodermal progenitor differentiation, spinal cord neurogenesis, somitogenesis, and forelimb bud initiation. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

10 Samples

Download data: BW

(Submitter supplied) Retinoic acid (RA) signaling plays a major role in controlling several developmental processes in vertebrate embryos. RA repression of caudal Fgf8 has emerged as a crucial mechanism through which RA controls body axis extension, somitogenesis, and spinal cord neurogenesis. The role of RA in Fgf8 repression is supported by mechanistic studies demonstrating direct RA repression through a nearby RA response element that recruits NCOR in an RA-dependent manner. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

2 Samples

Download data: XLSX

(Submitter supplied) The retinoic acid (RA) signaling pathway is essential for cardiac development; however, cardiomyocytes, the main cell type of the heart, are considered to be unresposive to RA signaling. The goal of this study was to examine the effects of all-trans RA (atRA) treatment on cardiomyocytes isolated from E18.5 mouse embryos. We found that cardiomyocytes, contrary to previous findings, are indeed repsonsive to RA signaling and that many genes involved in cardiac developmental and repair processes were significantly deregulated upon atRA trearment. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

8 Samples

Download data: XLSX

(Submitter supplied) The mouse aldehyde oxidase, Aoh2 (aldehyde oxidase homolog 2), is a molybdo-flavoenzyme. Harderian glands are the richest source of Aoh2, although the protein is detectable also in sebaceous glands, epidermis and other keratinized epithelia. The levels of Aoh2 in the Harderian gland and skin are controlled by genetic background, being maximal in CD1 or C57BL/6 and minimal in DBA/2, CBA or 129/Sv strains. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL2872 GPL7202

64 Samples

Download data: TXT

(Submitter supplied) Precise frequency discrimination is a hallmark of auditory function in birds and mammals. In the cochlea, tuning and spectral separation result from longitudinal differences in basilar membrane stiffness and numerous individual gradiations in sensory hair cell phenotypes, but it is unknown what patterns those phenotypes. Hypothesizing that morphogen levels might differ along the longitudinal axis of the developing cochlea, we sequenced the transcriptomes of the proximal, middle, and distal thirds of the chicken cochlea at E6.5, when postmitotic hair cells first form.

Organism:

Gallus gallus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16133

6 Samples

Download data: TXT

(Submitter supplied) Regulation of lineage specification and differentiation in embryonic stem (ES) cells can be achieved through the activation of endogenous signaling, an avenue for potential application in regenerative medicine. During vertebrate development, retinoic acid (RA) plays an important role in body axis elongation and mesoderm segmentation in that graded exposure to RA provides cells with positional identity and directs commitment to specific tissue lineages. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

4 Samples

Download data: CEL