GEO DataSets

Molecular basis of renewal properties in Bmi-1-deficient central and peripheral nervous system stem cells. Bmi-1 is a polycomb transcriptional repressor. Forebrain and gut neurospheres cultured from postnatal day 0 Bmi-1 -/- and wild type mice compared.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type, 2 strain sets

Platform:

GPL81

Series:

GSE611

4 Samples

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(Submitter supplied) The cRNA derived from Brain and Gut neurospheres of wild type and BMI-1 knockout mice were hybridized to Affymetrix mouse ver 2 Chips A, B and C. In case of brain neurospheres, the cRNA were generated by one round RNA amplification (conventional method). In contrast, the cRNA of Gut neurospheres were generated by two round RNA amplifications. Keywords: parallel sample

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS428 GDS429 GDS430

Platforms:

GPL81 GPL82 GPL83

12 Samples

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Molecular basis of renewal properties in Bmi-1-deficient central and peripheral nervous system stem cells. Bmi-1 is a polycomb transcriptional repressor. Forebrain and gut neurospheres cultured from postnatal day 0 Bmi-1 -/- and wild type mice compared.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type, 2 strain sets

Platform:

GPL83

Series:

GSE611

4 Samples

Download data

Molecular basis of renewal properties in Bmi-1-deficient central and peripheral nervous system stem cells. Bmi-1 is a polycomb transcriptional repressor. Forebrain and gut neurospheres cultured from postnatal day 0 Bmi-1 -/- and wild type mice compared.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type, 2 strain sets

Platform:

GPL82

Series:

GSE611

4 Samples

Download data

(Submitter supplied) Bmi1 is an important stem cell regulator in multiple tissues, including the lung. We used microarrays to analyze the dysregulation of gene expression in the absence of Bmi1 in the lung, to identify pathways important in Bmi1-dependent lung stem cell regulation

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4816

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

Analysis of primary lung cells FACS-isolated from Bmi1 deficient mutants. BMI1, a member of the Polycomb Repressive Complex 1, is required for the self-renewal of adult stem cells. Results provide insight into the mechanisms underlying the BMI1-dependent regulation of lung stem cell self-renewal.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE30852

6 Samples

Download data: CEL, CHP

(Submitter supplied) The RPMI-8226 human multiple myeloma cell line was stably infected with either a validated shRNA against BMI1 or a control shRNA. RNA was prepared from these lines, +/- doxycycline induction and at various time points post-induction. Samples were hybridized on the Affymetrix U133plus2 human genome expression microarray.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

42 Samples

Download data: CEL

(Submitter supplied) Paper abstract: Neural stem cells must strike a balance between self-renewal and multipotency, and differentiation. Identification of the transcriptional networks regulating stem cell division is an essential step in understanding how this balance is achieved. We have shown that the homeodomain transcription factor, Prospero, acts to repress self-renewal and promote differentiation. Amongst its targets are three neural stem cell transcription factors, Asense, Deadpan and Snail, of which Asense and Deadpan are repressed by Prospero. more...

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL9290

10 Samples

Download data: PAIR

(Submitter supplied) Paper abstract: Stem cells have the remarkable ability to give rise to both self-renewing and differentiating daughter cells. Drosophila neural stem cells segregate cell-fate determinants from the self-renewing cell to the differentiating daughter at each division. Here, we show that one such determinant, the homeodomain transcription factor Prospero, regulates the choice between stem cell self-renewal and differentiation. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL5135

12 Samples

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(Submitter supplied) The expression profile of asense mutant neuroblasts and GMCs were compared to wildtype in order to investigate the function of Asense in neural development. Abstract of paper: Neural stem cells must strike a balance between self-renewal and multipotency, and differentiation. Identification of the transcriptional networks regulating stem cell division is an essential step in understanding how this balance is achieved. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL5135

8 Samples

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(Submitter supplied) As Prdm16 deficiency reduces self-renewal potential and depletes neural stem cells in culture we decided to investigate the underlying molecular mechanisms of the neural stem cells depletion in the Prdm16 deficient animals. For the experiment we used Prdm16Gt(OST67423)Lex (Prdm16LacZ) genetrap mice obtained from the NIH Mutant Mouse Regional Resource Center (http://www.mmrrc.org/). We compared the gene expression profiles of uncultured ventricular zone cells from newborn Prdm16LacZ/LacZ (KO), Prdm16LacZ/+(HET), and Prdm16+/+ (WT) mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

9 Samples

Download data: CEL

(Submitter supplied) Progenitor cells maintain self-renewing tissues throughout life by sustaining their capacity for proliferation while suppressing cell cycle exit and terminal differentiation. DNA methylation provides a potential epigenetic mechanism for the cellular memory needed to preserve the somatic progenitor state through repeated cell divisions. DNA methyltransferase 1 (DNMT1) maintains DNA methylation patterns after cellular replication. more...

Organism:

Homo sapiens

Type:

Expression profiling by array; Methylation profiling by genome tiling array

Platforms:

GPL6325 GPL570 GPL6326

10 Samples

Download data: CEL, PAIR

(Submitter supplied) In the mouse neocortex, neural progenitor cells generate neurons through repeated rounds of asymmetric cell division. How distinct fates are established in their daughter cells is unclear. We show here that the TRIM-NHL protein TRIM32 segregates asymmetrically during progenitor division and induces neuronal differentiation in one of the two daughter cells. TRIM32 is highly expressed in differentiating neurons. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL7238

4 Samples

Download data: FNA, QUAL

(Submitter supplied) The purpose of this study is to assess the cellular heterogeneity within the buccal mucosal basal layer.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

2 Samples

Download data: MTX, TSV