|
| Status |
Public on Oct 01, 2018 |
| Title |
Single cell analysis of oral mucosa basal layer cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The purpose of this study is to assess the cellular heterogeneity within the buccal mucosal basal layer.
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| |
|
| Overall design |
Murine buccal mucosa was dissociated into a single cell suspension, and basal cells were FACS sorted based on their high level of CD104 (β4-integrin). Cells were barcoded using 10X genomics Chromium Strategy and 2 technical replicates were sequenced allowing us to look for specific sub-populations within the basal layer.
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| |
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| Contributor(s) |
Jones KB, Furukawa S, Marangoni P, Ma H, Pinkard H, D'Urso R, Zilionis R, Klein AM, Klein OD |
| Citation(s) |
30472156 |
| |
| Submission date |
Sep 28, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Pauline Anne Catherine Marangoni |
| E-mail(s) |
[email protected]
|
| Organization name |
UCSF
|
| Department |
OFS
|
| Lab |
Klein Lab
|
| Street address |
513 Parnassus Avenue, HSE15
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (2) |
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| Relations |
| BioProject |
PRJNA494028 |
| SRA |
SRP162965 |
