GEO DataSets

(Submitter supplied) Macrophages provide a crucial environment for Salmonella enterica serovar Typhi (S. Typhi) to multiply during typhoid fever, yet our understanding of how human macrophages and S. Typhi interact remains limited. In this study, we delve into the dynamics of S. Typhi replication within human macrophages and the resulting heterogeneous transcriptomic responses of macrophages during infection. Our study reveals key factors that influence macrophage diversity, uncovering distinct immune and metabolic pathways associated with different stages of S. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

18 Samples

Download data: TXT

(Submitter supplied) Six sequencing libraries was prepared from S. Typhi planktonic cells and biofilm cells using Illumina HiSeq 2500 sequencing to investigate differential gene expression between the two conditions. The transcriptome was processed using Cufflinks and there were a total of 35 up-regulated genes and 29 down-regulated genes log2-fold change values of greater than 2 and less than negative 2. The differentially expressed genes were identified using BLAST and the functions was analysed. more...

Organism:

Salmonella enterica subsp. enterica serovar Typhi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22930

6 Samples

Download data: CSV

(Submitter supplied) Typhoid fever, caused primarily by Salmonella enterica serovar Typhi (S. Typhi), is a life-threatening systemic disease responsible for significant morbidity and mortality worldwide. 3-5% of individuals infected with S. Typhi become chronic carriers due to bacterial persistence in the gallbladder. We have demonstrated that Salmonella forms biofilms on gallstones to establish gallbladder carriage. However, an in-depth molecular understanding of chronic carriage in the gallbladder, from the perspective of both the pathogen and host, is poorly defined. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

13 Samples

Download data: XLSX

(Submitter supplied) Global expression profiles of Salmonella typhi grown in the supernatant of infection and within human macrophages at 0h, 2h, 8h and 24h were obtained. Stringent analytical methods were used to compare Salmonella typhi cDNAs and revealed that known virulence factors, such as the SPI-1 and SPI-2 encoded type III secretion systems, were found to be expressed as predicted during infection by Salmonella. more...

Organism:

Salmonella enterica; Salmonella; Salmonella enterica subsp. enterica serovar Typhi

Type:

Expression profiling by array

Platforms:

GPL548 GPL1835

30 Samples

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(Submitter supplied) The cDNA obtained before and after each round of SCOTS (first, second and third) from 2 h post-infection were hybridized to analyse the effect of SCOTS round on transcripts population. The number of detected genes increase with the numer of round of SCOTS. Because the cDNA prepared by 3 round of SCOTS gave the best results, three round of SCOTS were used in the preparation of the other samples. Keywords: Evaluation of SCOTS round

Organism:

Salmonella enterica; Salmonella enterica subsp. enterica serovar Typhi

Type:

Expression profiling by array

Platform:

GPL548

24 Samples

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(Submitter supplied) Genomic DNA from Strain ISP1820 and CT18 were compared to identify missing genes in strain ISP1820. Keywords: Comparative Genomic Hybridization

Organism:

Salmonella enterica subsp. enterica serovar Typhi; Salmonella enterica

Type:

Genome variation profiling by array

Platform:

GPL548

3 Samples

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(Submitter supplied) We were able to obtain distinct gene expression profile between children with typhoid fever and other bacteremic infections.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

6 Samples

Download data: TXT

(Submitter supplied) We applied an RNA capture and amplification technique, Selective Capture of Transcribed Sequences (SCOTS), and microarray hybridization to identify S. Typhi transcripts expressed in the blood of five humans infected with S. Typhi in Bangladesh. In total, we detected the expression of mRNAs for 2046 S. Typhi genes (44% of the S. Typhi genome) in human blood; expression of 912 genes was detected in all 5 patients, and expression of 1100 genes was detected in 4 or more patients. more...

Organism:

Salmonella enterica; Salmonella enterica subsp. enterica serovar Typhi

Type:

Expression profiling by array

Platform:

GPL13884

20 Samples

Download data: TXT