GEO DataSets

(Submitter supplied) Sclerotinia sclerotiorum is a pathogenic fungus that infects hundreds of crop species, causing extensive yield loss every year. Chemical fungicides are used to control this phytopathogen, but with concerns about increasing resistance and impacts on non-target species, there is a need to develop alternative control measures. In the present study, we engineered Brassica napus to constitutively express a hairpin (hp)RNA molecule to silence ABHYRDOLASE-3 in S. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26890

18 Samples

Download data: XLSX

(Submitter supplied) In this study, we developed transgenic Arabidopsis thaliana (AT1703) expressing double-stranded (ds)RNA to silence S. sclerotiorum ABHYDROLASE-3 and slow infection through host induced gene silencing (HIGS). Leaf infection assays show reduced S. sclerotiorum lesion size, fungal load, and ABHYDROLASE-3 transcript abundance in AT1703 lines compared to wild-type Col-0. To better understand how HIGS influences host-pathogen interactions, we performed global RNA sequencing on AT1703 and wild-type Col-0 lines directly at the site of S. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21785

17 Samples

Download data: TXT

(Submitter supplied) Global transcriptome profiling of suceptible and tolerant lines of Brassica napus infected with Sclerotinia sclerotiorum using a petal inoculation method that mimics field conditions.

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

12 Samples

Download data: GTF, XLSX

(Submitter supplied) RNA-seq of Sclerotinia sclerotiorum-infected B. napus leaves during a timecourse after inoculation.

Organism:

Sclerotinia sclerotiorum; Brassica napus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL22102 GPL22103

21 Samples

Download data: XLSX

(Submitter supplied) Background: The biological control agent Pseudomonas chlororaphis PA23 is effective at protecting Brassica napus (canola) from the necrotrophic fungus Sclerotinia sclerotiorum via direct antagonism. Despite the growing importance of biocontrol bacteria in plant protection from fungal pathogens, little is known about how the host plant responds to bacterial priming on the leaf surface or about changes in gene activity genome-wide in the presence and absence of S. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

12 Samples

Download data: DIFF, FPKM_TRACKING, TXT

(Submitter supplied) The fungal pathogen Sclerotinia sclerotiorum infects a broad range of dicotyledonous plant species and is the causative agent of stem rot in Brassica napus. To elucidate the mechanisms underlying the defense response, we studied the patterns of gene expression in a partially resistant variety of ZhongYou 821 (ZY821) and a susceptible line from Westar over five time points, 6, 12, 24, 48, and 72 hours post-inoculation (hpi) using a B. more...

Organism:

Brassica napus

Type:

Expression profiling by array

Platform:

GPL7499

60 Samples

Download data: TXT

(Submitter supplied) Quantitative disease resistance (QDR) is a form of plant immunity conserved across species able to limit infections caused by a broad range of pathogens. QDR has a complex genetic determinism, the bases of which are not fully understood. The number of genes contributing to the QDR response and the extent to which molecular components of the QDR response vary across plant species remain elusive. The fungal pathogen Sclerotinia sclerotiorum, causal agent of white mold disease on hundreds of plant species, triggers QDR responses in host populations. more...

Organism:

Helianthus annuus; Beta vulgaris subsp. vulgaris; Phaseolus vulgaris; Ricinus communis

Type:

Expression profiling by high throughput sequencing

4 related Platforms

24 Samples

Download data: TXT

(Submitter supplied) Cooperation is associated with major transitions in evolution such as the emergence of multicellularity. It is central to the evolution of many complex traits in nature, including growth and virulence in pathogenic bacteria. Whether cells of multicellular parasites function cooperatively during infection remains however largely unknown. Here, we show that hyphal cells of the fungal pathogen Sclerotinia sclerotiorum reprogram towards division of labor to facilitate the colonization of host plants. more...

Organism:

Sclerotinia sclerotiorum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20037

15 Samples

Download data: TXT

(Submitter supplied) Plant pathogens with a broad host range are able to infect plant lineages that diverged over 100 million years ago. They exert similar and recurring constraints on the evolution of unrelated plant populations. Plants generally respond with quantitative disease resistance (QDR), a form of immunity relying on complex genetic determinants. In most cases, the molecular determinants of QDR and how they evolve is unknown. more...

Organism:

Solanum lycopersicum; Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19694 GPL17639

12 Samples

Download data: TXT

(Submitter supplied) Secreted laccases are important enzymes on an ecological scale for their role in mediating plant-fungal interactions, but their function in fungal pathogenesis has yet to be elucidated. Ascomycete laccases have been primarily associagted with cell wall melanin deposition, and laccase mutants in ascomycete species often demonstrate reduced pigmentation. In this study, a putatively secreted laccase, Sslac2, was characterized from the broad host-range plant pathogen Sclerotinia sclerotiorum, which is largely unpigmented and is not dependent on melanogenesis for plant infection. more...

Organism:

Sclerotinia sclerotiorum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32607

12 Samples

Download data: TXT