Similar studies for GEO DataSets (Select 200152703) - GEO DataSets

Select item 2001527031.

Transcript profiling of putative MTF-1 activating chemicals in wild-type and MTF-1-null MCF-7 cells

(Submitter supplied) In future high-throughput transcriptomic studies, we propose that the use of cell lines nullizygous for important targets of environmental chemicals will greatly facilitate the interpretation of regulated genes. To provide proof of this concept, we generated transcript profiles from wild-type and MTF-1-null cells exposed to zinc (Zn) and six putative activators of MTF-1 generated using TempO-Seq targeted sequencing of ~3000 human genes. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
48 Samples

Download data: TXT

Series

Accession:: GSE152703

ID:: 200152703

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 2000765102.

Zinc sensing by metal-responsive transcription factor 1 (MTF1) controls metallothionein and ZnT1 expression to buffer the sensitivity of the transcriptome response to zinc

(Submitter supplied) Only a small number of genes are known direct targets of the zinc-responsive transcription factor MTF1; therefore, the aim of this study was to gain a more complete understanding of the MTF-1 regulated zinc-responsive component of the transcriptome. A targeted siRNA was used to deplete MTF1 expression in the human intestinal cell line Caco-2. We predicted that the response to zinc of direct MTF1 target genes would be abrogated by MTF1 knockdown. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL10558
12 Samples

Download data: TXT

Series

Accession:: GSE76510

ID:: 200076510

Select item 2000405353.

Genome-wide dMTF-1-binding sites in Drosophila S2 cells in the absence or presence of copper or cadmium

(Submitter supplied) Cells respond to changes in environment by shifting their gene expression profile to deal with the new conditions. The cellular response to changes in metal homeostasis is an important example of this. Transition metals such as iron, zinc, and copper are essential micronutrients but other metals such as cadmium are simply toxic. The cell must maintain metal concentrations in a window that supports efficient metabolic function but must also protect against the damaging effects of high concentrations of these metals. more...

Organism:: Drosophila melanogaster

Type:: Genome binding/occupancy profiling by genome tiling array

Platform:: GPL6629
3 Samples

Download data: BAR, CEL

Series

Accession:: GSE40535

ID:: 200040535

PubMed Full text in PMC Similar studies

Select item 2001536164.

Transcript profiling of putative NF-kB activators in wild-type and NFKB1-null HeLa cells

(Submitter supplied) In future high-throughput transcriptomic studies, we propose that the use of cell lines nullizygous for important target genes of environmental chemicals will greatly facilitate the interpretation of gene expression profiles. To provide support for this concept, we generated transcript profiles of known or putative NF-kB activators in wild-type and NFKB1-null HeLa cells. Both cell lines were treated with interleukin 1 (IL1beta), tumor necrosis alpha (TNFalpha) or one of two chemicals identified in high throughput assays for NF-kB activity (chlorhexidine diacetate and carbocyanine). more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
30 Samples

Download data: TXT

Series

Accession:: GSE153616

ID:: 200153616

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Select item 2001090215.

Identification of Androgen Receptor Modulators in a Prostate Cancer Cell Line Microarray Compendium

(Submitter supplied) High-throughput transcriptomic (HTTr) technologies are increasingly being used to screen environmental chemicals in vitro to identify molecular targets and provide mechanistic context for regulatory testing. The androgen receptor (AR, NR3C4) regulates male sexual development, is involved in the pathogenesis of a number of cancers, and is often the target of endocrine disruptors. Here, we describe the development and validation of a novel gene expression biomarker to identify AR-modulating chemicals using a pattern matching method. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL10558
96 Samples

Download data: IDAT

Series

Accession:: GSE109021

ID:: 200109021

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Select item 2000512986.

Inhibition of endogenous MTF-1 signaling in zebrafish embryos identifies novel roles for MTF-1 in development

(Submitter supplied) The MTF-1 transcription factor is considered to be a master regulator of zinc homoestasis. We previously characterized a constitutively nuclear, dominant-negative zebrafish MTF-1/eGFP fusion protein (dnMTF-1). In this study, in vitro transcribed dnMTF-1 mRNA was microinjected into zebrafish embryos (2-cell stage), with controls consisting of zebrafish embryos (2-cell stage) microinjected with in vitro transcribed enhanced green fluorescent protein (eGFP) mRNA. more...