GEO DataSets

(Submitter supplied) We previously established the transcription factor Zfp423 is critical for maintaining white adipocyte identity through suppression of the thermogenic gene program. The loss of Zfp423 in mature adipocytes triggers the rapid conversion of energy-storing white adipocytes into thermogenic beige adipocytes in subcutaneous WAT. In contrast to subcutaneous WAT, visceral WAT is relatively resistant to browning. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

24 Samples

Download data: XLSX

(Submitter supplied) We derived a model that allows for doxycycline-inducible deletion of Zfp423 in mature adipocytes of adult mice (Adiponectin-rtTA; TRE-CRE; Zfp423 loxP/loxP). In these animals deletion of Zfp423 results in a spontaneous conversion of white adipocytes into beige-like adipocytes at room temperature. The goal of this expression analysis was to 1) determine the gene programs dependent on adipocyte Zfp423 in inguinal WAT, and 2) determine the similarity between the converted beige-like cells to normal beige adipose tissue that accumulates upon cold exppsure.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20775

9 Samples

Download data: CEL

(Submitter supplied) We investigated gene expression signatures in subcutaneous inguinal adipose tissue obtained from wild type and R6/2 mice with the aim to identify gene expression changes and signalling pathway alterations in adipose tissue relevant to HD. Gene expression was assessed using Affymetrix GeneChip® Mouse Gene 2.0 ST Array. Target genes were technically validated using real-time quantitative PCR.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

12 Samples

Download data: CEL, CHP

(Submitter supplied) Obesity is a risk factor for numerous metabolic disorders; however, not all obese individuals are prone to insulin resistance. The central aim of this study was to identify molecular pathways directly related to insulin resistance independent of BMI in obesity. We sought to determine the gene expression signature of adipose tissue in a body mass index (BMI)-matched obese cohort of patients that are either insulin sensitive or insulin resistant.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3781

Platform:

GPL570

39 Samples

Download data: CEL

Analysis of subcutaneous and visceral adipose tissue from body mass index (BMI)-matched, obese patients who were insulin-sensitive versus insulin-resistant, thereby eliminating obesity as a variable. Results provide insight into molecular mechanisms mediating obesity-related insulin resistance.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state, 2 gender, 2 tissue sets

Platform:

GPL570

Series:

GSE20950

39 Samples

Download data: CEL

(Submitter supplied) Effects of YBX1 activation in PPARγ-indcuded C3H/10T1/2-SAM pre-adipocytes on the transcriptome of cells during early differentation stages

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

51 Samples

Download data: TSV

(Submitter supplied) We found the mPRAT adipose undergoes a whitning process which differs from iBAT and iWAT. The whitened adipocytes can still respond to cold exposure, which has cellular and molecular differences compared with iBAT and iWAT.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

12 Samples

Download data: MTX, TSV

(Submitter supplied) Visceral white adipose tissue is closed correlated with obesity and metabolic dysfunction. Epididymal adipose tissue (eWAT) is considered as typical visceral white adipose tissue. Induction of browning of white adipose tissue improves metabolic dysfunction such as insulin resistance. In contrast to mice subcutaneous adipose tissue, visceral fat do not show significant browning under 4°C. However,under physiologically tolerable low temperature visceral adipose tissue can turn brown. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6096

12 Samples

Download data: CEL

(Submitter supplied) The induction of beige/brite adipose cells in white adipose tissue (WAT) is associated with protection against high fat diet-induced obesity and insulin resistance in animals. The helix-loop-helix transcription factor Early B-Cell Factor-2 (EBF2) regulates brown adipose tissue development. We examined the role of EBF2 in beige fat cell biogenesis by comparing transcriptome in wildtype and EBF2-overexpressing mice in the adipose tissue.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

8 Samples

Download data: XLS

(Submitter supplied) Comparing gene expression profiles of murine subcutaneous vs. visceral adipose tissue. Gene expression was analyzed in two subcutaneous depots (inguinal and axillary) and two visceral depots (epididymal and mesenteric) from male C57Bl/6 mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5654

Platform:

GPL8321

12 Samples

Download data: CEL

Analysis of adipose tissue from two subcutaneous depots (inguinal and axillary) and two visceral depots (epididymal and mesenteric) from age-matched, C57Bl/6 males. SubQ and VISC represent two broad categories of obesity. Results provide insight into fat depot-specific molecular profiles.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 other, 2 tissue sets

Platform:

GPL8321

Series:

GSE53307

12 Samples

Download data: CEL

(Submitter supplied) Beige and brown adipocytes generate heat in response to reductions in ambient temperature. When warmed, both beige and brown adipocytes exhibit morphological ‘whitening’, but it is unknown whether or to what extent this represents a true shift in cellular identity. Using cell type-specific profiling in vivo, we uncover a unique paradigm of temperature-dependent epigenomic plasticity of beige, but not brown, adipocytes, with conversion from a brown to a white chromatin state. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL17021 GPL19057

58 Samples

Download data: BW, TSV

(Submitter supplied) We analyzed coding and noncoding transcript abundance in primary differentiating brite adipocytes derived from murine inguinal white adipose tissue, 24 hours in response to lncRNA Ctcflos knockdown at day 1 of differentiation

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

6 Samples

Download data: TXT

(Submitter supplied) We analyzed coding and noncoding transcript abundance in primary differentiating brite adipocytes derived from murine inguinal white adipose tissue, 24 hours or 72 hours in response to lncRNA Ctcflos knockdown at day 1 of differentiation

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

24 Samples

Download data: TXT

(Submitter supplied) Single cell sequencing technologies are providing unexpected insights on how seemingly homogenous cell populations differ markedly in their functional properties, and how diverse cell repertoires mediate the functions of tissues and organs. Adipose tissue controls multiple key aspects of systemic energy homeostasis, but only two human adipocyte subtypes have been recognized so far. Here we developed methods to characterize single human mesenchymal progenitors and discovered four previously unknown adipocyte subtypes specialized for distinct adipose tissue functions, which are derived from distinct progenitors. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

156 Samples

Download data: TXT

(Submitter supplied) Activation and recruitment of thermogenic cells in human white adipose tissues (“browning”) can counteract obesity and associated metabolic disorders. However, quantifying the effects of therapeutic interventions on browning remains enigmatic. Here, we devise a computational approach, profiling of fat tissue types (ProFAT), for the quantification of thermogenic potential of heterogeneous fat biopsies based on the prediction of white and brown adipocytes content from raw gene expression profiles. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

16 Samples

Download data: TXT

(Submitter supplied) Mechanisms that control “beige/brite” thermogenic adipose tissue development may be harnessed to improve human metabolic health. To define these mechanisms, we developed a species-hybrid model in which human mesenchymal progenitor cells were used to develop white or thermogenic/beige adipose tissue in mice. The hybrid adipose tissue developed distinctive features of human adipose tissue, such as larger adipocyte size, despite its neurovascular architecture being entirely of murine origin. more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24625

7 Samples

Download data: CSV

(Submitter supplied) Epidydimal WAT from adipose tissue Pgam1 deficient mice shows the phenotype of beiging. We used single cell RNA sequencing (scRNA-seq) to characterize the beiging cells in gWAT.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21493

2 Samples

Download data: MTX, TSV

(Submitter supplied) To analyze the gene expression profile of BAT and gWAT from Pgam1 depletion mice, we performed whole genome microarray expression profiling using brown adipose tissue (BAT) and gonadal white adipose tissue (gWAT) from adipose tissue-specific Pgam1 knockout (KO) mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL21163

16 Samples

Download data: TXT

(Submitter supplied) Brown adipose tissue can expend large amounts of energy and thus increasing its amount or activity is a promising therapeutic approach to combat metabolic disease. In humans, major deposits of brown fat cells are found intimately associated with large blood vessels, corresponding to perivascular adipose tissue (PVAT). However, the cellular origins of PVAT are poorly understood. We applied single cell transcriptomic analyses, ex vivo adipogenesis assays, and genetic fate mapping to determine the identity of perivascular adipocyte progenitors. more...

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21103 GPL24676

6 Samples

Download data: MTX, RDS, TSV