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| Status |
Public on Mar 19, 2021 |
| Title |
Transcriptome changes in differentiating primary brite adipocytes 24 hours after knockdown of long noncoding RNA Ctcflos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We analyzed coding and noncoding transcript abundance in primary differentiating brite adipocytes derived from murine inguinal white adipose tissue, 24 hours in response to lncRNA Ctcflos knockdown at day 1 of differentiation
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| Overall design |
We prepared primary preadipocytes from stromal-vascular fraction of murine inguinal white adipose tissue of 129SvEv mice, induced them at sub-confluence for 2 days and differentiated them for 1 day in the presence of rosiglitazone to induce browning. Cells were reverse transfected with lncRNA Ctcflos-targeting or non-targeting control LNA Gapmer Antisense oligonucleotides [50nM] and harvested either 24 hours post transfection. We deep sequenced total RNA and determined transcript abundance.
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| Contributor(s) |
Bast-Habersbrunner A, Li Y, Tobias F, Klingenspor M |
| Citation(s) |
34056831 |
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| Submission date |
Mar 18, 2021 |
| Last update date |
Jun 18, 2021 |
| Contact name |
Tobias Fromme |
| E-mail(s) |
[email protected]
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| Organization name |
Technical University of Munich
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| Department |
Molecular Nutritional Medicine
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| Street address |
Gregor-Mendel-Str. 2
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| City |
Freising |
| ZIP/Postal code |
85354 |
| Country |
Germany |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA715440 |
| SRA |
SRP311203 |
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