GEO DataSets

(Submitter supplied) Background: In the event of an improvised nuclear device detonation, the prompt radiation exposure would consist of γ rays plus a neutron component that would contribute to the total dose. As neutrons cause more complex and difficult to repair damage to cells that would result in more severe health burden to affected individuals, it is paramount to be able to estimate the contribution of neutrons to an estimated dose, to provide information for those making treatment decisions. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10333

72 Samples

Download data: TXT

(Submitter supplied) Background: Radiation exposure due to the detonation of an improvised nuclear device remains a major security concern. Radiation from such a device involves a combination of photons and neutrons. Although photons will make the greater contribution to the total dose, neutrons will certainly have an impact on the severity of the exposure as they have high relative biological effectiveness. Results: We investigated the gene expression signatures in the blood of mice exposed to 3 Gy x-rays, 0.75 Gy of neutrons, or to mixed field photon/neutron with the neutron fraction contributing 5%, 15%, or 25% of a total 3 Gy radiation dose. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

36 Samples

Download data: TXT

(Submitter supplied) The detonation of an improvised nuclear device would produce prompt radiation consisting of both photons (gamma-rays) and neutrons. While much effort in recent years has gone into the development of radiation biodosimetry methods suitable for mass triage, the possible effect of neutrons on the endpoints studied has remained largely uninvestigated. We have used a novel neutron irradiator with an energy spectrum based on that 1-1.5 km from the epicenter of the Hiroshima blast to begin examining the effect of neutrons on global gene expression, and the impact this may have on the development of gene expression signatures for radiation biodosimetry. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

92 Samples

Download data: TXT

(Submitter supplied) In the current global scenario there is always a possibility that a terrorist group might acquire material to produce a nuclear device. In the likelihood of a detonation of an improvised nuclear device (IND) the prompt radiation would consist of both photons (gamma rays) and neutrons. As neutrons generally have a high Relative Biological Effectiveness (RBE) for most physiological endpoints, it is important to understand the impact that neutrons would have on the biodosimetry methods that are being developed for medical triage purposes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

30 Samples

Download data: TXT

(Submitter supplied) Background: Ionizing Radiation (IR) is a known pro-inflammatory agent and in the process of development of biomarkers for radiation biodosimetry a chronic inflammatory disease condition could act as a confounding factor. Hence, it is important to develop radiation biodosimetry that can distinguish between IR-induced inflammatory responses and pre-existing disease. In this study, we compared the gene expression response of a genetically modified mouse model of inflammatory bowel disease (Il10-/-) with that of a normal wild-type mouse to potentially develop transcriptomic based biodosimetry markers for individual susceptibility to radiation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

20 Samples

Download data: TXT

(Submitter supplied) Due to limitations of available human models for development of gene expression based radiation biodosimetry, many such studies have made use of mouse models. To provide a broad view of the gene expression response to irradiation in the mouse, we have exposed male C57BL/6 mice to 0, 1.5, 3, 6 or 10 Gy of gamma rays, sacrificing groups of the mice at 1, 2, 3, 5, or 7 days after exposure. Global gene expression was then profiled in blood from individual mice using Agilent microarrays. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

248 Samples

Download data: TXT

(Submitter supplied) This study was designed to identify molecular changes induced by radiation in mouse lung.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10333

9 Samples

Download data: TXT

(Submitter supplied) Background: In the event of an improvised nuclear device or radiological dirty bomb detonation large numbers of people will be exposed to radiation that would require a timely and accurate biodosimetry to identify the highly exposed individuals who would require medical treatment from those who have received low or no radiation (the “worried well”). Gene expression signatures in response to radiation have been derived from mice and human peripheral blood and the impact of a number of variables, such as dose, time, genotype, and gender, on gene expression has been analyzed. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

24 Samples

Download data: TXT

(Submitter supplied) [original title] Microarray analysis of DNA damage repair gene expression profiles in cervical cancer cells radioresistant to 252Cf neutron and X-rays. The aim of the study was to obtain stable radioresistant sub-lines from the human cervical cancer cell line HeLa by prolonged exposure to 252Cf neutron and X-rays. Radioresistance mechanisms were investigated in the resulting cells using SuperArray Oligo GEArray® Human DNA Damage Signaling Pathway Microarray.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL9811

3 Samples

Download data: TXT

(Submitter supplied) Background: The effects of dose-rate and its implications on radiation biodosimetry methods are not well studied in the context of large-scale radiological scenarios. There are significant health risks to individuals exposed to an acute dose in such an event, but the most realistic scenario would be a combination of exposure to both high and low dose-rates, from both external and internal radioactivity. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

35 Samples

Download data: TXT

(Submitter supplied) Radiation biodosimetry based on transcriptomic analysis of peripheral blood is a valuable tool to detect radiation exposure after a radiological/nuclear event and obtain useful biological information that could predict tissue and organismal injury. However, confounding factors, including inflammation, can potentially obscure the predictive power of the method. The Gadd45 (growth arrest and DNA damage-inducible genes) family is composed of three members, Gadd45a, Gadd45b, and Gadd45g, that play important roles in cell growth control, differentiation, DNA repair, and apoptosis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

24 Samples

Download data: TXT

(Submitter supplied) Our in vivo study measures miRNA and gene expression changes in human blood cells in response to ionizing radiation in order to develop miRNA signatures that can be used as biomarkers for radiation exposure. Blood was collected from 8 radiotherapy patients immediately prior to and at 4 h after total body irradiation with 1.25 Gy X-rays. Both miRNA and gene expression changes were measured by means of quantitative PCR and microarray hybridization, respectively. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

16 Samples

Download data: TXT

(Submitter supplied) In the event of a large-scale radiation exposure incident, accurate and quick assessment of radiation dose would be critical for triage and medical treatment of large numbers of potentially exposed individuals. Current methods of biodosimetry, such as the dicentric chromosome assay, are time consuming and require sophisticated equipment and highly trained personnel. Therefore, scalable biodosimetry approaches, including gene expression profiles in peripheral blood cells, are being investigated. more...

Organism:

Macaca mulatta; Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10332

167 Samples

Download data: TXT

(Submitter supplied) The objective of this investigation was to characterize, at individual level, the transcriptional response and the onset of regenerative processes in mouse skin irradiated with different doses of fast neutrons. We performed a high-throughput gene expression analysis, by DNA oligonucleotide microarray on 24 three months old C57Bl/6 mice irradiated with 0, 0,2 and 1 Gy of mono-energetic 14 MeV neutron. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11211

8 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL16027

72 Samples

Download data: TXT

(Submitter supplied) This is an experiment to study the effects of total body irradiation on intestinal gene expression. There are 24 male animals in the study; 4 controls, 10 animals received 6.7 Gy, 10 received 7.4 Gy. Animals were killed 4, 7 or 12 days after irradiation

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL16027

24 Samples

Download data: TXT

(Submitter supplied) This is an experiment to study the effects of total body irradiation on intestinal gene expression. There are 24 male animals in the study; 4 controls, 10 animals received 6.7 Gy, 10 received 7.4 Gy. Animals were killed 4, 7 or 12 days after irradiation

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL16027

24 Samples

Download data: TXT

(Submitter supplied) This is an experiment to study the effects of total body irradiation on intestinal gene expression. There are 24 male animals in the study; 4 controls, 10 animals received 6.7 Gy, 10 received 7.4 Gy. Animals were killed 4, 7 or 12 days after irradiation

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL16027

24 Samples

Download data: TXT

(Submitter supplied) There is a perceived need to develop tools for high-throughput radiation biodosimetry. In the case of an improvised nuclear device or a dirty bomb in a major city, potentially hundreds of thousands of people would have to be screened to assign exposed individuals to appropriate treatment. Gene expression represents an emerging approach to biodosimetry and could potentially provide an estimate of both absorbed dose and individual radiation-induced injury. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

32 Samples

Download data: TXT

(Submitter supplied) Adopting boron neutron capture, we separately irradiated Kupffer cells and endothelial cells in mouse liver in vivo and the changes in gene transcriptions were analyzed by an oligonucleotide microarray. Keywords: Gene expression

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL5978 GPL5984

8 Samples

Download data: TXT