GEO DataSets

(Submitter supplied) Global transcriptome profiling of suceptible and tolerant lines of Brassica napus infected with Sclerotinia sclerotiorum using a petal inoculation method that mimics field conditions.

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

12 Samples

Download data: GTF, XLSX

(Submitter supplied) Background: The biological control agent Pseudomonas chlororaphis PA23 is effective at protecting Brassica napus (canola) from the necrotrophic fungus Sclerotinia sclerotiorum via direct antagonism. Despite the growing importance of biocontrol bacteria in plant protection from fungal pathogens, little is known about how the host plant responds to bacterial priming on the leaf surface or about changes in gene activity genome-wide in the presence and absence of S. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

12 Samples

Download data: DIFF, FPKM_TRACKING, TXT

(Submitter supplied) RNA-seq of Sclerotinia sclerotiorum-infected B. napus leaves during a timecourse after inoculation.

Organism:

Brassica napus; Sclerotinia sclerotiorum

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL22102 GPL22103

21 Samples

Download data: XLSX

(Submitter supplied) The fungal pathogen Sclerotinia sclerotiorum infects a broad range of dicotyledonous plant species and is the causative agent of stem rot in Brassica napus. To elucidate the mechanisms underlying the defense response, we studied the patterns of gene expression in a partially resistant variety of ZhongYou 821 (ZY821) and a susceptible line from Westar over five time points, 6, 12, 24, 48, and 72 hours post-inoculation (hpi) using a B. more...

Organism:

Brassica napus

Type:

Expression profiling by array

Platform:

GPL7499

60 Samples

Download data: TXT

(Submitter supplied) Sclerotinia sclerotiorum is a pathogenic fungus that infects hundreds of crop species, causing extensive yield loss every year. Chemical fungicides are used to control this phytopathogen, but with concerns about increasing resistance and impacts on non-target species, there is a need to develop alternative control measures. In the present study, we engineered Brassica napus to constitutively express a hairpin (hp)RNA molecule to silence ABHYRDOLASE-3 in S. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26890

18 Samples

Download data: XLSX

(Submitter supplied) The hemibiotrophic fungal pathogen Leptosphaeria maculans is the causal agent of blackleg disease in Brassica napus (canola, oilseed rape) and causes significant losses in crop yields worldwide. While genetic resistance has been used to mitigate the disease, little information about the genes and gene regulatory networks underlying blackleg resistance is currently available. High-throughput RNA sequencing and rigorous bioinformatics approaches revealed dynamic changes in the host transcriptome and identified plant defense pathways specific to the host-pathogen incompatible LepR1-AvrLepR1 interaction.

Organism:

Plenodomus lingam; Brassica napus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19731 GPL21443

36 Samples

Download data: XLSX

(Submitter supplied) The goal of the microarray experiment was to identify defense genes that were differentially expressed in the Arabidopsis mutants med16, med8, elp2, wild type in response to infection of the necrotrophic fungal pathogen Sclerotinia sclerotiorum. Results indicated that, compared with the wild type, the jasmonic acid (JA) biosynthesis genes LOX3, AOC3, and OPR3, ethylene (ET) biosynthesis genes ACS2 and ACS8, as well as salicylic acid (SA) biosynthesis genes ICS1 and EDS5 were up-regulated in med16 in local tissues at 1 day post-inoculation (dpi) and/or systemic tissues at 4 dpi. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL12621

48 Samples

Download data: TXT