GEO DataSets

(Submitter supplied) Acute myeloid leukemia (AML) is characterized by a marked genetic heterogeneity, which complicates the development of novel therapeutics. The delineation of pathways essential within the patient-individual mutational background might overcome this limitation and facilitate personalized treatment. We report the results of a large-scale lentiviral loss-offunction RNA-interference-(RNAi)-screen in primary leukemic cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome variation profiling by high throughput sequencing; Other

Platform:

GPL11154

10 Samples

Download data: TXT, VCF

(Submitter supplied) Poor clinical outcome of Acute Myeloid Leukemia (AML) and Myelodysplastic Syndrome (MDS) has been attributed to failure of current chemotherapeutic regimens to target leukemic stem cells. We recently identified p21-activated kinase (PAK1) as a downstream effector molecule of H2.0-like homeobox (HLX), a gene functionally relevant for AML pathogenesis. In this study, we find that inhibition of PAK1 activity by small molecule inhibitors or by RNA interference leads to profound leukemia-inhibitory effects both in vitro and in vivo. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

8 Samples

Download data: CEL, CHP

(Submitter supplied) Mononuclear cells from AML patients (n=46) were sorted into CD34+ and CD34- subfractions and genome-wide expression analysis was performed using Illumina BeadChip Arrays (HT12 v3). Of 2 AML samples only the CD34+ fraction could be analyzed. AML CD34+ and CD34- gene expression was compared to a large group of normal CD34+ bone marrow cells (n=31).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6947

121 Samples

Download data: TXT

(Submitter supplied) Analysis of genome wide gene expression changes over a time course of 24 hours induced by the Rho kinase inhibitor Fasudil in primary mouse astrocyte cultures.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3944

Platform:

GPL6885

32 Samples

Download data: TXT

Analysis of astrocytes treated with Rho kinase (ROCK) inhibitor fasudil for up to 24 hrs. ROCK inhibitors inhibit glial scarring, a reactive process involving astrogliosis that occurs after injury to CNS. Results provide mechanistic insights into beneficial action of fasudil during brain injury.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent, 4 time sets

Platform:

GPL6885

Series:

GSE25829

32 Samples

Download data

(Submitter supplied) Transcriptional profiling of KASUMI-1 cells comparing between control (DMSO-treated) and radotinib treated Kasumi-1 cells at 48hr

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

1 Sample

Download data: TXT

(Submitter supplied) Rho-associated kinase (ROCK) and zipper-interacting protein kinase (ZIPK) have been implicated in diverse physiological functions, including smooth muscle contraction, cell proliferation, cell adhesion, apoptosis, cell migration and inflammation. Many aspects of regulation via ROCK and ZIPK, however, remain unclear. In this study, we utilized an siRNA approach to knock down ROCK1 and ZIPK in cultured human arterial smooth muscle cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS5659 GDS5948

Platform:

GPL6244

9 Samples

Download data: CEL

Analysis of cultured vascular smooth muscle cells following knockdown of zipper-interacting protein kinase (ZIPK). ZIPK is phosphorylated and activated by Rho-associated kinase 1 (ROCK1). These results, together with those from GDS5659, provide further insight into ROCK1 and ZIPK functions.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL6244

Series:

GSE56819

6 Samples

Download data: CEL

Analysis of cultured vascular smooth muscle cells following knockdown of Rho-associated kinase 1 (ROCK1). ROCK1 phosphorylates and activates zipper-interacting protein kinase (ZIPK). These results, together with those from GDSxxxx, provide further insight into ROCK1 and ZIPK functions.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL6244

Series:

GSE56819

6 Samples

Download data: CEL