GEO DataSets

(Submitter supplied) Neuronal cultures were treated with candesartan at neuroprotective concentrations followed by excitotoxic glutamate amounts. Candesartan significantly reduced glutamate-induced inflammation. To provide mechanistic insight into the potential targets and pathways that may underlie these benefits, we performed genome wide expression profile analysis and evaluated the data by Ingenuity Pathway Analysis (IPA) and Gene Set Enrichment Analysis (GSEA). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

16 Samples

Download data: CEL, CHP

(Submitter supplied) We investigate the direct effects of ARBs on prostate cancer growth and progression.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL4135

8 Samples

Download data: TXT

(Submitter supplied) To increase our understanding of the mechanisms that underlie neuroprotection following glutamate receptor-induced excitotoxicity, we treated primary CD-1 mouse cortical neurons with NMDA in the presence of absence of neuroprotective compounds. The results indicate a convergence at the level of whole genome expression, despite the divergent chemistries and purported mechanisms of action. While more arrays were performed than published, only the extreme arrays were used in subsequent analyses.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6105

48 Samples

Download data: TXT

(Submitter supplied) Alzheimer's disease (AD) is a devastating neurodegenerative disorder that threatens to reach epidemic proportions as our population ages. Although much research has examined molecular pathways associated with AD, relatively few studies have focused on critical early stages. Our prior microarray study correlated gene expression in human hippocampus with AD markers. Results suggested a new model of early-stage AD in which pathology spreads along myelinated axons, orchestrated by upregulated transcription and epigenetic factors related to growth and tumor suppression (Blalock et al., 2004). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4136

Platform:

GPL570

30 Samples

Download data: CEL

Analysis of laser-captured hippocampal CA1 gray matter from FFPE hippocampal sections of subjects at varying stages (incipient, moderate, severe) of Alzheimer’s disease (AD). Results provide insight into gray matter-specific molecular mechanisms underlying AD.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 19 age, 4 disease state sets

Platform:

GPL570

Series:

GSE28146

30 Samples

Download data: CEL

(Submitter supplied) CAD-31 has potent neuroprotective properties in six distinct nerve cell assays that mimic toxicities observed in the old brain. Pharmacological and preliminary toxicology studies show that CAD-31 is brain penetrant and likely safe. When fed to old, symptomatic transgenic Alzheimer's disease (AD) mice starting at ten months of age for three additional months in a therapeutic model of the disease, there was a reduction in the memory deficit and brain inflammation, and an increase in the expression of synaptic proteins. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

13 Samples

Download data: TXT

(Submitter supplied) Gene expression profiling reveals a potential role of Verbenalin in neural induction and neuronal differentiation of hAECs. hAECs were isolated from discarded term placenta and were treated with 20 μM verbenalin for seven days. Microarray gene expression profiling was conducted for biological replicates of RA-treated (T7) and untreated control cells on day 0 (D0) and day 7 (D7).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

8 Samples

Download data: CEL, CHP

(Submitter supplied) High levels of oxidative stress and an associated neuronal DDR occur at the earliest stages of Alzheimer pathology (low Braak stage), and is associated with cognitive impairment. The aim of the present study was to combine immuno-LCM and microarray analysis to characterize the neuronal transcriptome at low Braak stages (Braak 0-II), with respect to the neuronal DDR, in post-mortem human frontal cortex derived from the Medical Research Council Cognitive Function and Ageing Study (MRC-CFAS).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

8 Samples

Download data: CEL

(Submitter supplied) Multiple cell types and complex connection networks are an intrinsic feature of brain tissue. In this study we used expression profiling of homogeneous cell populations from specific microscopic regions of heterogeneous tissue sections isolated by laser capture microdissection (LCM) to determine insights into the molecular basis of brain pathology in prion disease. Temporal profiles in two mouse models of prion disease, bovine spongiform encephalopathy (BSE) and a mouse-adapted strain of scrapie (RML) were performed in microdissected regions of the CA1 and cerebellar granular that are enriched in neuronal cell bodies. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

76 Samples

Download data: TXT

(Submitter supplied) Alzheimer’s disease (AD) is the most common form of dementia but has no effective treatment. A comprehensive investigation of cell-type-specific responses and cellular heterogeneity in AD is required to provide precise molecular and cellular targets for therapeutic development. Accordingly, we perform single-nucleus transcriptome analysis of 169,496 nuclei from the prefrontal cortical samples of AD patients and normal control (NC) subjects. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

21 Samples

Download data: MTX, TSV

(Submitter supplied) We used Illumina Human HT-12 v4 arrays to compare RNA expression of middle temporal gyrus (MTG; BA21) in Alzheimer’s Disease (AD = 97) and non-demented controls (ND = 98). A total of 938 transcripts were highly differentially expressed (adj p < 0.01; log2 Fold Change (FC) ≥ |0.500|, with 411 overexpressed and 527 underexpressed in AD. Our results correlated with expression profiling in neurons from AD and ND obtained by Laser Capture Microscopy in MTG from an independent dataset (log2 FC correlation: r = 0.504; p = 2.2e-16). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

195 Samples

Download data: TXT, XLSX

(Submitter supplied) Purpose: To gain a better understanding of the molecular mechanisms through which candesartan improves revocery after controlled cortical impact. Methods: We performed a transcriptomic analysis of the ipsilateral hippocampus, thalamus, and hypothalamus at 3 and 29 days post-injury utilizing bulk mRNA-seq. Results: A majority of the differentially expressed genes were identified in the hippocampus at 3 days post injury showing alterations in angiogenesis, interferon signaling, extracellular matrix organization, and DNA replication. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21493

96 Samples

Download data: TXT

(Submitter supplied) Clinical trials of novel therapeutics for Alzheimer's Disease (AD) have consumed a large amount of time and resources with largely negative results. Repurposing drugs already approved by the Food and Drug Administration (FDA) for another indication is a more rapid and less expensive option. In this study, we profile 80 FDA-approved and clinically tested drugs in neural cell cultures, with the goal of producing a ranked list of possible repurposing candidates.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

764 Samples

Download data: CSV

(Submitter supplied) Type 2 diabetes mellitus (T2D), characterised by peripheral insulin resistance, is a risk factor for dementia. In addition to its contribution to small and large vessel disease, T2D may directly damage cells of the brain neurovascular unit. In this study, we investigated the transcriptomic changes in cortical neurones, and associated astrocytes and endothelial cells of the neurovascular unit, in the ageing brain

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

33 Samples

Download data: CEL

(Submitter supplied) Prolactin (Prl) is a pleiotropic hormone with multiple functions in several tissues and organs, including the brain. In the hippocampus, Prl has been implicated in several functions, including neuroprotection against excitotoxicity in lactating rats and in Prl-treated ovariectomized animals. However, the molecular mechanisms involved in Prl actions in the hippocampus have not been completely elucidated. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20084

12 Samples

Download data: TXT

(Submitter supplied) 10x single-cell RNA-sequencing to profile human cerebrovascular cells in health and disease

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

25 Samples

Download data: TAR

(Submitter supplied) We report here the bacTRAP (bacterial artificial chromosome , translating ribosome affinity purification) profiling of 7 different types of neurons in the mouse, at three different ages: two neuron types very vulnerable to AD (principal cells of entorhinal cortex layer II - ECII), pyramidal cells of hippocampus CA1, and 5 types of neurons more resistant to AD (pyramidal cells of hippocampus CA2 and CA3, granule neurons of the dentate gyrus, pyramidal cells from layer IV of primary visual cortex V1, and pyramidal cells from layer II/III and V of primary somatosensory cortex S1). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

122 Samples

Download data: TXT

(Submitter supplied) Purpose: To identify gene expression changes in entorhinal cortex layer II (ECII) neurons upon Ptbp1 modulation (silencing and overexpression) Method: bacterial artificial chromosome - Translating Ribosome Affinity Purification (bacTRAP) to isolate actively translated mRNA in ECII neurons, 2 weeks after stereotaxic injection of an AAV1 vector in the EC of ECII-bacTRAP mice; followed by RNAseq. Note: Ptbp1 was significantly overexpressed in the overexpression experiment, but no silencing was achieved with the silencing vector, probably because of tight control of Ptbp1 expression

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

18 Samples

Download data: TXT

(Submitter supplied) The recent advances in creating pluripotent stem cells from somatic cells and differentiating them into a variety of cell types is allowing us to study them without the caveats associated with disease-related changes. We generated induced Pluripotent Stem Cells (iPSCs) and used lentiviral delivery to differentiate them into excitatory glutamatergic neurons, on which we performed RNA sequencing. We compared transcriptomes of cells derived from 7 Alzheimer’s disease (AD) and 6 control patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

13 Samples

Download data: CSV

(Submitter supplied) Study on selective vulnerability of certain brain regions to oxidative stress. Here we selected 4 brain regions (hippocampal CA1 and CA3, cerebral cortex, and cerebellar granular layer) to study this phenomenon. Keywords: Comparative analysis of different regions of the brain.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3726

Platform:

GPL341

12 Samples

Download data: CEL