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| Status |
Public on May 29, 2020 |
| Title |
RNAseq anaysis of entorhinal cortex layer II neurons upon Ptbp1 modulation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To identify gene expression changes in entorhinal cortex layer II (ECII) neurons upon Ptbp1 modulation (silencing and overexpression) Method: bacterial artificial chromosome - Translating Ribosome Affinity Purification (bacTRAP) to isolate actively translated mRNA in ECII neurons, 2 weeks after stereotaxic injection of an AAV1 vector in the EC of ECII-bacTRAP mice; followed by RNAseq. Note: Ptbp1 was significantly overexpressed in the overexpression experiment, but no silencing was achieved with the silencing vector, probably because of tight control of Ptbp1 expression
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| Overall design |
in IP162 (silencing), 5 mice injected with a scrambled-mcherry vector, 5 with a Ptbp1 shRNA-mcherry vector; 1 Ptbp1 shRNA mouse was excluded because no read mapped to mcherry; in IP170 (overexpression), 5 mice injected with control-mcherry vector, 5 mice with a Ptbp1-mcherry vector; 1 control mouse was excluded because no read mapped to mcherry; in both experiments, the two entorhinal cortices were pooled
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| Contributor(s) |
Roussarie J |
| Citation(s) |
32603655 |
| |
| Submission date |
May 28, 2020 |
| Last update date |
Aug 28, 2020 |
| Contact name |
Jean-Pierre Roussarie |
| E-mail(s) |
[email protected]
|
| Organization name |
Boston University School of Medicine
|
| Department |
Anatomy & Neurobiology
|
| Street address |
72 E Concord Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA635608 |
| SRA |
SRP265083 |
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