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| Status |
Public on Jun 02, 2015 |
| Title |
Neuronal DNA damage response-associated dysregulation of signalling pathways and cholesterol metabolism at th earliest stages of Alzheimer-type pathology |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
High levels of oxidative stress and an associated neuronal DDR occur at the earliest stages of Alzheimer pathology (low Braak stage), and is associated with cognitive impairment.
The aim of the present study was to combine immuno-LCM and microarray analysis to characterize the neuronal transcriptome at low Braak stages (Braak 0-II), with respect to the neuronal DDR, in post-mortem human frontal cortex derived from the Medical Research Council Cognitive Function and Ageing Study (MRC-CFAS).
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| Overall design |
Pyramidal neurones were isolated from age, sex and brain pH-matched cases derived from the MRC-CFAS cohort, using immuno laser capture microdissection. The extracted RNA was amplified and applied to HGU133 Plus 2.0 Affymetrix gene arrays. Genes were considered differentially expressed at p<0.001 in all 4 individual cases in each DDR group [8 cases in total: 4 cases high neuronal DDR & 4 cases low neuronal DDR].
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| Contributor(s) |
Simpson JE, Ince PG, Minett T, Matthews FE, Heath PR, Shaw PJ, Goodall E, Garwood CJ, Ratcliffe LE, Brayne C, Rattray M, Wharton SB |
| Citation(s) |
26095650 |
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| Submission date |
Feb 26, 2015 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Paul Roy Heath |
| E-mail(s) |
[email protected]
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| Phone |
+44 114 2222254
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| Organization name |
University of Sheffield
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| Department |
Academic Neurology Unit
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| Lab |
Heath
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| Street address |
SITraN, 385a Glossop Road
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| City |
Sheffield |
| State/province |
S. Yorks |
| ZIP/Postal code |
S10 2HQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| GSM1619929 |
Frontal cortex pyramidal neurons, high DNA damage response 1 |
| GSM1619930 |
Frontal cortex pyramidal neurons, high DNA damage response 2 |
| GSM1619931 |
Frontal cortex pyramidal neurons, high DNA damage response 3 |
| GSM1619932 |
Frontal cortex pyramidal neurons, high DNA damage response 4 |
| GSM1619933 |
Frontal cortex pyramidal neurons, low DNA damage response 1 |
| GSM1619934 |
Frontal cortex pyramidal neurons, low DNA damage response 2 |
| GSM1619935 |
Frontal cortex pyramidal neurons, low DNA damage response 3 |
| GSM1619936 |
Frontal cortex pyramidal neurons, low DNA damage response 4 |
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| Relations |
| BioProject |
PRJNA276559 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66333_RAW.tar |
35.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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