GEO DataSets

(Submitter supplied) This study used TaqMan low-density arrays to identify and quantitate circulating cellular miRNAs during HIV-1 elite suppression, active HIV-1 replication, and uninfected status.

Organism:

Homo sapiens

Type:

Other

Platform:

GPL14842

18 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array; Other

Platforms:

GPL14822 GPL14842

39 Samples

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(Submitter supplied) This study used the NanoString nCounter hybridization system and nCounter miRNA expression assays to identify and quantitate circulating cellular miRNAs during HIV-1 elite suppression, active HIV-1 replication, and uninfected status.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL14822

21 Samples

Download data: TXT

(Submitter supplied) qRT-PCR TaqMan Low-Density Array Protocol: see manufacturer's website: http://www.appliedbiosystems.com/

Organism:

Homo sapiens

2 Series

18 Samples

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(Submitter supplied) hybridization array with capture probes and fluorescently barcoded detection probes Protocol: see manufacturer's website: http://www.nanostring.com/

Organism:

Homo sapiens

2 Series

21 Samples

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(Submitter supplied) Background: The emerging relationship between microRNAs (miRNA) and viral-control is a topic of interest in the field of HIV. Host-genome might play an important role in the control of viremia. The aim of this study was to assess the specific miRNA profile that could contribute to the control of HIV replication in Elite Controllers Results: After adequate normalization, expression profile of 286 human miRNAs (hsa-miR) was evaluated in phytohaemagglutinin-stimulated PBMCs from 29 individuals classified in 4 groups: 8 elite controllers (EC; viral load <50 cp/ml without treatment), 8 viremic progressors (VP; VL>5000 cp/ml without treatment), 8 patients under antiretroviral treatment (ART; VL<200cp/ml) and 5 uninfected individuals (HIV-) through TaqMan® Array Human microRNA Cards v3.0. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL17391

29 Samples

Download data: TXT

(Submitter supplied) Assessment of the extent to which the altered profiles of miRNA expression influence viral replication and latency, as well as the efficiency of host defenses, may be useful for understanding the basis of the HIV-1-related alterations in cellular physiology and immunologic control. To this end, three patient groups were enrolled. One group consisted of subjects who were classified as élite control long-term non-progressors (éLTNP). more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL14851

24 Samples

Download data: TXT

(Submitter supplied) HIV-1 elite controllers maintain undetectable levels of viral replication in the absence of antiretroviral therapy, but their underlying immunological and virological characteristics may vary. Here, we used a whole-genome transcriptional profiling approach to characterize gene expression signatures of CD4 T cells from an unselected cohort of elite controllers. In the majority of elite controllers, transcriptional profiles were similar to HAART-treated patients, while being different from HIV-1 negative persons. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8432

40 Samples

Download data: TXT

(Submitter supplied) There is great interindividual variability in HIV-1 viral set point after seroconversion, some of which is known to be due to genetic differences among infected individuals. Here, our focus is on determining, genome-wide, the contribution of variable gene expression to viral control, and to relate it to genomic DNA polymorphism. RNA was extracted from purified CD4+ T-cells from 137 HIV-1 seroconverters, 16 elite controllers, and 3 healthy blood donors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6884

202 Samples

Download data: TXT

(Submitter supplied) The virus-specific CD4+ T cell dysfunction associated with failure to control chronic infections is poorly understood in humans. In this study we sought to determine transcriptional signatures that link HIV-specific T cell help to viral control by performing genome wide transcriptional analysis on ex vivo stimulated HIV-specific CD4+ T cells from HIV-infected subjects with diverse viral loads, who were not on antiretroviral therapy at time of sampling.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23126

32 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23126

81 Samples

Download data: CEL

(Submitter supplied) The virus-specific CD4+ T cell dysfunction associated with failure to control chronic infections is poorly understood in humans. An issue of critical clinical relevance is the lack of restoration of effective anti-HIV immunity after suppressive ART: viral rebound is the rule after cessation of therapy. Whether persistent HIV-specific CD4+ T cell dysfunction on ART contribute to this failed response is an important, yet unresolved, question. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23126

32 Samples

Download data: CEL

(Submitter supplied) The virus-specific CD4+ T cell dysfunction associated with failure to control chronic infections is poorly understood in humans. A striking enrichment of a Tfh signature is observed in ex vivo stimulated HIV-specific CD4 T cells of Chronic progressors (CPs) compared to Elite controls (ECs), which was not due to a higher fraction of CXCR5mem HIV-specific CD4 in CPs. Here, we sought to define the cell subsets that contributed to the TFH transcriptional signature observed in CPs, applying microarray analysis to paired live-sorted subsets of CXCR5mem and CXCR5neg HIV-specific CD4+ T cells from CPs and ECs . more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23126

17 Samples

Download data: CEL

(Submitter supplied) Changes in miRNA profile of CD4+ T cells treated with cocaine

Organism:

Rattus norvegicus; Mus musculus; Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL17075

3 Samples

Download data: GPR

(Submitter supplied) The question of how HIV-1 interfaces with cellular microRNA (miRNA) biogenesis and effector mechanisms has been highly controversial. Here, we first used deep sequencing of small RNAs present in two different infected cell lines (TZM-bl and C8166) and two types of primary human cells (CD4+ PBMCs and macrophages) to unequivocally demonstrate that HIV-1 does not encode any viral miRNAs. Perhaps surprisingly, we also observed that infection of T cells by HIV-1 has only a modest effect on the expression of cellular miRNAs at early times after infection. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing; Other

Platform:

GPL11154

14 Samples

Download data: BED

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL14907 GPL6947

24 Samples

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(Submitter supplied) Host cells respond to exogenous infectious agents such as viruses, including HIV-1. Studies have evaluated the changes associated with virus infection at the transcriptional and translational levels of the cellular genes involved in specific pathways. While this approach is useful, in our view it provides only a partial view of genome-wide changes. Recently, technological advances in the expression profiling at the microRNA (miRNA) and mRNA levels have made it possible to evaluate the changes in the components of multiple pathways. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6947

12 Samples

Download data: TXT

(Submitter supplied) Host cells respond to exogenous infectious agents such as viruses, including HIV-1. Studies have evaluated the changes associated with virus infection at the transcriptional and translational levels of the cellular genes involved in specific pathways. While this approach is useful, in our view it provides only a partial view of genome-wide changes. Recently, technological advances in the expression profiling at the microRNA (miRNA) and mRNA levels have made it possible to evaluate the changes in the components of multiple pathways. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL14907

12 Samples

Download data: XLS

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL24676

18 Samples

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(Submitter supplied) CD4+ T-cells are the main target of HIV-1 and several host factors can positively or negatively modulate HIV-1 infection of these cells. MiRNAs aresmall regulatory RNAs that are involved in the regulation of basic cellular functions. They are also increasingly recognized as host factors regulatingHIV-1 infection, replication and persistence. In order to identify miRNAs involved in HIV-1 infection of CD4+ T-cells, we performed globaltranscriptomic analyses of productively infected and HIV-1 exposed but non infected bystander CD4+ T-cells and compared their MIRNA profiles with uninfected cells. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL24676

9 Samples

Download data: XLSX