GEO DataSets

(Submitter supplied) Citrus greening or huanglongbing (HLB) is a devastating disease of citrus. HLB is associated with the phloem-limited fastidious prokaryotic alpha-proteobacterium Candidatus Liberibacter spp. In this report, we used sweet orange (Citrus sinensis) leaf tissue infected with 'Ca. Liberibacter asiaticus' and compared this with healthy controls. Investigation of the host response was examined with citrus microarray hybridization based on 30,171 sets expressed sequence tag sequences from several citrus species and hybrids. more...

Organism:

Citrus sinensis

Type:

Expression profiling by array

Platform:

GPL5731

6 Samples

Download data: CEL

(Submitter supplied) HLB is suggested to be caused by the phloem-limited fastidious prokaryotic α-proteobacterium “Candidatus Liberibacter spp.” Previous studies focused on the proteome and transcriptome analyses of citrus 5 to 35-week-after “Ca. L. spp.” inoculation. In this study, gene expression profiles was analyzed using mandarin of Citrus reticulate Blanco cv. jiaogan leaves after 2-year infection with “Ca. L. asiaticus”.

Organism:

Citrus sinensis; Citrus reticulata

Type:

Expression profiling by array

Platform:

GPL5731

2 Samples

Download data: CEL

(Submitter supplied) Candidatus Liberibacter asiaticus infection of citrus is characterized by symptom variability within and among organs. In order to identify molecular processes involved in the regulation of organ response to Ca. Liberibacter infection, the gene expression patterns in C. sinensis leaf, stem, and root was examined in Affymetrix microarray. Our analyses showed that Ca. L. asiaticus reprograms several cellular and metabolic processes in C. more...

Organism:

Citrus sinensis

Type:

Expression profiling by array

Platform:

GPL5731

12 Samples

Download data: CEL

(Submitter supplied) Candidatus Liberibacter asiaticus (Las), a non-culturable phloem-limited bacterium, is the suspected causal agent of Huanglongbing (HLB) in Florida. HLB is one of the most devastating diseases of citrus and no resistant cultivars have been identified to date, though tolerance has been suggested in the genus Poncirus. A recent study conducted in our laboratory demonstrated tolerance of US-897, a hybrid of Poncirus trifoliata (L. more...

Organism:

Citrus sinensis; Citrus reticulata; Citrus reticulata x Citrus trifoliata

Type:

Expression profiling by array

Platform:

GPL5731

12 Samples

Download data: CEL

(Submitter supplied) In this data set, we reported for the first time that huanglongbing disease (HLB) induces major changes in the expression of global genes in flavedo, vascular and juice vesicle tissues of citrus fruit.

Organism:

Citrus sinensis

Type:

Expression profiling by array

Platform:

GPL5731

67 Samples

Download data: CEL

(Submitter supplied) We evaluated the effect on citrus trees of two newly-identified molecules, benzbromarone and tolfenamic acid, used as antimicrobials in commercial groves of sweet orange (Citrus sinensis). We delivered the molecules by trunk injection and evaluated safety and efficacy parameters by performing RNAseq of the citrus host responses.

Organism:

Citrus sinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21082

52 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

42 Samples

Download data: CEL

(Submitter supplied) We characterized the transcriptome of a Sinorhizobium meliloti visNR deletion mutant (MB1102) expressing Ca. Liberibacter asiaticus visNR

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

6 Samples

Download data: CEL

(Submitter supplied) We characterized transcriptomes of a Sinorhizobium meliloti rpoH1rpoH2 deletion mutant (RFF231; Lang et al. 2018, mSphere 3:e00454-18) expressing either Ca. Liberibacter asiaticus rpoH or Sinorhizobium meliloti rpoH1

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

9 Samples

Download data: CEL

(Submitter supplied) We characterized the transcriptome of a Sinorhizobium meliloti phrR deletion mutant (MB1142) expressing Ca. Liberibacter asiaticus phrR

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

6 Samples

Download data: CEL

(Submitter supplied) We characterized the transcriptome of a Sinorhizobium meliloti lsrB deletion mutant (MB1105) expressing Ca. Liberibacter asiaticus lsrB

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

6 Samples

Download data: CEL

(Submitter supplied) We characterized the transcriptome of a Sinorhizobium meliloti ldtR deletion mutant (MB1101) expressing Ca. Liberibacter asiaticus ldtR

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

6 Samples

Download data: CEL

(Submitter supplied) We characterized transcriptomes of a Sinorhizobium meliloti wild type strain (CL150) expressing either Ca. Liberibacter asiaticus ctrA or Sinorhizobium meliloti ctrA

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

9 Samples

Download data: CEL

(Submitter supplied) Root samples of ‘Sanhu’ red tangerine trees infected with and without Candidatus Liberibacter asiaticus (CLas) were collected at 50 days post inoculation and subjected to RNA-sequencingto profile the differentially expressed genes (DEGs) . Results showed that a total of 3956 genes were differentially regulated by HLB-infection. Comparison between our results and those of the previously reported showed that known HLB-modulated biological pathways including cell-wall modification, protease-involved protein degradation, carbohydrate metabolism, hormone synthesis and signaling, transcription activities, and stress responses were similarly regulated by HLB infection but different or root-specific changes did exist. more...

Organism:

Citrus reticulata

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19990

2 Samples

Download data: GFF3, TXT

(Submitter supplied) Candidatus Liberibacter asiaticus (Las) is an emergent bacterial pathogen associated with the devastating citrus Huanglongbing (HLB), also known as the greening disease. Vectored by the Asian Citrus Psyllid (Diaphorina citri), Las colonizes the phloem tissue of citrus. So far, efforts of cultivating Las in vitro have not been successful and dual-transcriptome analyses could only detect ~100 Las genes due to the low abundance of bacterial RNA in infected citrus/psyllid tissues. more...

Organism:

Citrus x paradisi; Citrus macrophylla; Diaphorina citri

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL30420 GPL30421 GPL30419

22 Samples

Download data: TXT

(Submitter supplied) Huanglongbing (HLB) is a worldwide devastating disease of citrus. There are no effective control measures for this newly emerging but century-old disease. Previously, we reported a combination of Penicillin G and Streptomycin was effective in eliminating or suppressing the associated bacterium, ‘Candidatus Liberibacter asiaticus’ (Las). Here we report the bacterial composition and community structure in HLB-affected citrus plants during a growing season and while being treated with antibiotic combinations PS (Penicillin G and Streptomycin) and KO (Kasugamycin and Oxytetracycline) using the Phylochip™ G3 array.

Organism:

Citrus sinensis; Archaea; Bacteria

Type:

Other

Platform:

GPL17136

9 Samples

Download data: CEL

(Submitter supplied) The goals of this study are to reveal the transcriptional and regulatory dynamics of the lncRNAs in response to CLas infection and provide a reference for a better understanding of the role of lncRNAs involved in citrus HLB regulation. For the purpose, we obtained RNA-seq-based differential expression profile of the leave midrib of CLas- and mock-inoculated rough lemon and sweet orange at week 0, 7, 17, and 34 following infections with three biological replicates in the greenhouse. more...

Organism:

Citrus jambhiri; Citrus sinensis

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL17145 GPL29550

48 Samples

Download data: FA, TXT

(Submitter supplied) RNA sequencing of L. crescens grown in different stress conditions.

Organism:

Liberibacter crescens BT-1

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30517

8 Samples

Download data: TXT

(Submitter supplied) Many virus diseases of economic importance to agriculture result from mixtures of different pathogens invading the host at a given time. This contrasts with the relatively scarce studies available on the molecular events associated with virus-host interactions in mixed infections. In comparison to single infections, co-infection of Nicotiana benthamiana with Potato virus X (PVX) and Potato virus Y (PVY) resulted in increased systemic symptoms (synergism) that led to necrosis of the newly emerging leaves, and the plant death. more...

Organism:

Nicotiana benthamiana; Solanum tuberosum

Type:

Expression profiling by array

Platform:

GPL3838

9 Samples

Download data: GPR

(Submitter supplied) The Atss3 mutant and WT plants were arranged according to a Randomized Complete Block Design. The plants were planted in rows with seven rows in each flat; two plants of the same genotype/pot. Plants were grown under a SD photoperiod (8 h light/16 h dark) in a growth chamber as described. Eight randomly selected rows were harvested for each time point from different flats. Plant material was harvested at five time points in the diurnal cycle (1, 4, 8.5, 12, and 16 h; Time 0 is the beginning of the light period); harvesting was conducted under a green safety light. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

20 Samples

Download data: CEL, CHP