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| Status |
Public on Jun 12, 2008 |
| Title |
Global gene expression in Atss3 mutant and WT over short day diurnal cycle |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The Atss3 mutant and WT plants were arranged according to a Randomized Complete Block Design. The plants were planted in rows with seven rows in each flat; two plants of the same genotype/pot. Plants were grown under a SD photoperiod (8 h light/16 h dark) in a growth chamber as described. Eight randomly selected rows were harvested for each time point from different flats. Plant material was harvested at five time points in the diurnal cycle (1, 4, 8.5, 12, and 16 h; Time 0 is the beginning of the light period); harvesting was conducted under a green safety light. Each sample consisted of rosette leaves (leaves 5 to 8, staged following Bowmann (1994); photosynthetically active (Stessman et al., 2002)) from sixteen six-week-old plants. Leaf samples were frozen in liquid N2 immediately after harvest and stored at -80‚°C for RNA extraction. The experiment was done twice and independent randomizations for plant growth and harvest were used for the two replicates.
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| Overall design |
The Atss3 mutant and WT plants were arranged according to a Randomized Complete Block Design. The plants were planted in rows with seven rows in each flat; two plants of the same genotype/pot. Plants were grown under a SD photoperiod (8 h light/16 h dark) in a growth chamber as described. Eight randomly selected rows were harvested for each time point from different flats. Plant material was harvested at five time points in the diurnal cycle (1, 4, 8.5, 12, and 16 h; Time 0 is the beginning of the light period); harvesting was conducted under a green safety light. Each sample consisted of rosette leaves (leaves 5 to 8, staged following Bowmann (1994); photosynthetically active (Stessman et al., 2002)) from sixteen six-week-old plants. Leaf samples were frozen in liquid N2 immediately after harvest and stored at -80°C for RNA extraction. The experiment was done twice and independent randomizations for plant growth and harvest were used for the two replicates.
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| Contributor(s) |
Li L, Wurtele ES |
| Citation(s) |
19154206 |
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| Submission date |
Jun 07, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ling Li |
| E-mail(s) |
[email protected]
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| Phone |
515-294-6236
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| Organization name |
Iowa State University
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| Department |
Department of Genetics, Development, and Cell Biology
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| Lab |
Eve Wurtele
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| Street address |
443 Bessey Hall
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| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (20)
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA106003 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11708_RAW.tar |
146.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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