GEO DataSets

(Submitter supplied) The objective of this investigation was to characterize, at individual level, the transcriptional response and the onset of regenerative processes in mouse skin irradiated with different doses of fast neutrons. We performed a high-throughput gene expression analysis, by DNA oligonucleotide microarray on 24 three months old C57Bl/6 mice irradiated with 0, 0,2 and 1 Gy of mono-energetic 14 MeV neutron. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11211

8 Samples

Download data: GPR

(Submitter supplied) Background: In the event of an improvised nuclear device detonation, the prompt radiation exposure would consist of γ rays plus a neutron component that would contribute to the total dose. As neutrons cause more complex and difficult to repair damage to cells that would result in more severe health burden to affected individuals, it is paramount to be able to estimate the contribution of neutrons to an estimated dose, to provide information for those making treatment decisions. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10333

72 Samples

Download data: TXT

(Submitter supplied) Background: Radiation exposure due to the detonation of an improvised nuclear device remains a major security concern. Radiation from such a device involves a combination of photons and neutrons. Although photons will make the greater contribution to the total dose, neutrons will certainly have an impact on the severity of the exposure as they have high relative biological effectiveness. Results: We investigated the gene expression signatures in the blood of mice exposed to 3 Gy x-rays, 0.75 Gy of neutrons, or to mixed field photon/neutron with the neutron fraction contributing 5%, 15%, or 25% of a total 3 Gy radiation dose. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

36 Samples

Download data: TXT

(Submitter supplied) Expression profiles in mouse liver exposed to long-term gamma-irradiation were examined to assess in vivo effects of low dose-rate radiation. Three groups of male C57BL/6J mice were exposed to whole body irradiation at dose-rates of 17-20 mGy/day, 0.86-1.0 mGy/day or 0.042-0.050 mGy/day for 401-485 days (cumulative doses were approximately 8 Gy, 0.4 Gy or 0.02 Gy, respectively). Expression profiles were produced for RNA isolated from irradiated individual animals and for pooled RNA from sham-irradiated 3 animals for control. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

20 Samples

Download data: CEL, CHP

(Submitter supplied) The detonation of an improvised nuclear device would produce prompt radiation consisting of both photons (gamma-rays) and neutrons. While much effort in recent years has gone into the development of radiation biodosimetry methods suitable for mass triage, the possible effect of neutrons on the endpoints studied has remained largely uninvestigated. We have used a novel neutron irradiator with an energy spectrum based on that 1-1.5 km from the epicenter of the Hiroshima blast to begin examining the effect of neutrons on global gene expression, and the impact this may have on the development of gene expression signatures for radiation biodosimetry. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

92 Samples

Download data: TXT

(Submitter supplied) [original title] Microarray analysis of DNA damage repair gene expression profiles in cervical cancer cells radioresistant to 252Cf neutron and X-rays. The aim of the study was to obtain stable radioresistant sub-lines from the human cervical cancer cell line HeLa by prolonged exposure to 252Cf neutron and X-rays. Radioresistance mechanisms were investigated in the resulting cells using SuperArray Oligo GEArray® Human DNA Damage Signaling Pathway Microarray.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL9811

3 Samples

Download data: TXT

(Submitter supplied) This study was designed to identify molecular changes induced by radiation in mouse lung.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10333

9 Samples

Download data: TXT

(Submitter supplied) Transcriptomic profiling of normal mouse kidney cortex and medulla following 177Lu irradiation

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

72 Samples

Download data: TXT

(Submitter supplied) Due to limitations of available human models for development of gene expression based radiation biodosimetry, many such studies have made use of mouse models. To provide a broad view of the gene expression response to irradiation in the mouse, we have exposed male C57BL/6 mice to 0, 1.5, 3, 6 or 10 Gy of gamma rays, sacrificing groups of the mice at 1, 2, 3, 5, or 7 days after exposure. Global gene expression was then profiled in blood from individual mice using Agilent microarrays. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

248 Samples

Download data: TXT

(Submitter supplied) Background: In the event of an improvised nuclear device or radiological dirty bomb detonation large numbers of people will be exposed to radiation that would require a timely and accurate biodosimetry to identify the highly exposed individuals who would require medical treatment from those who have received low or no radiation (the “worried well”). Gene expression signatures in response to radiation have been derived from mice and human peripheral blood and the impact of a number of variables, such as dose, time, genotype, and gender, on gene expression has been analyzed. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

24 Samples

Download data: TXT

(Submitter supplied) Increased use of nuclear reactors and radioactive materials for energy production and proliferation of nuclear weaponry increases risk of radiation exposure or nuclear accidents. Development of radiation countermeasures for the diagnosis, prognosis, and radiation treatment are trailing behind, and knowledge of the deleterious radiation effects on health and responses to exposure at the molecular, cellular, and systems biology level are still not fully understood. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

82 Samples

Download data: TXT