GEO DataSets

(Submitter supplied) affy_ralstonia_medicago - Ralstonia solanacearum is the causal agent of the devastating bacterial wilt disease. Its infection process was studied with an in vitro inoculation procedure on intact roots of Medicago truncatula. The pathosystem involved susceptible A17 and resistant F83005.5 M truncatula lines infected with the pathogenic strain GMI1000. The mutant A17 line, Sickle, which showed a resistant phenotype was also part of the experiment. more...

Organism:

Medicago sativa; Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL4652

27 Samples

Download data: CEL

(Submitter supplied) In order to better understand the commonalities and differences in lateral root and nodule development, we compared their organogenesis and correlated this with changes in gene expression. To initiate lateral roots in Medicago truncatula we turned 2-day-old seedlings 135°, before returning them to their original axis of growth, while for nodule initiation we applied droplets of Sinorhizobium meliloti on the susceptibility zone of the root.

Organism:

Medicago truncatula

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21643

240 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sinorhizobium meliloti; Medicago sativa; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4652

114 Samples

Download data: CEL

(Submitter supplied) Legumes interact with soil fungi, leading to the development of arbuscular mycorrhizal (AM) roots. Diffusible AM fungal signals were identified as sulphated and non-sulphated LCOs (sMyc-LCOs and nsMyc-LCOs). Applying Myc-LCOs on roots of symbiotic mutants, we used GeneChips to detail the global programme of gene expression in these mutants in response to the external application of Myc-LCOs. Keywords: Expression profiling by array

Organism:

Sinorhizobium meliloti; Medicago sativa; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4652

48 Samples

Download data: CEL

(Submitter supplied) Legumes interact with rhizobia, leading to the development of root nodules. Diffusible rhizobial signals were identified as Nod-LCOs. Applying Nod-LCOs on plantlet roots, we used GeneChips to detail the global programme of gene expression in response to the external application of Nod-LCOs.

Organism:

Medicago sativa; Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL4652

30 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sinorhizobium meliloti; Medicago sativa; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4652

60 Samples

Download data: CEL

(Submitter supplied) Legumes interact with soil fungi, leading to the development of arbuscular mycorrhizal (AM) roots. Diffusible AM fungal signals were identified as sulphated and non-sulphated LCOs (sMyc-LCOs and nsMyc-LCOs). Applying Myc-LCOs on roots of symbiotic mutants, we used GeneChips to detail the global programme of gene expression in these mutants in response to the external application of Myc-LCOs.

Organism:

Medicago sativa; Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL4652

24 Samples

Download data: CEL

(Submitter supplied) Legumes interact with soil microbes, leading to the development of nitrogen-fixing root nodules and arbuscular mycorrhizal (AM) roots. While nodule initiation by diffusible lipochitooligosaccharide (LCO) Nod-factors of bacterial origin (Nod-LCOs) is well characterized, diffusible AM fungal signals were only recently identified as sulphated and non-sulphated LCOs (sMyc-LCOs and nsMyc-LCOs). Applying Myc-LCOs in parallel to Nod-LCOs, we used GeneChips to detail the global programme of gene expression in response to the external application of symbiotic LCOs.

Organism:

Medicago sativa; Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL4652

36 Samples

Download data: CEL

(Submitter supplied) The extend of chromatin accessibility changes and its consequent impact on the transcriptional control of rhizobial infection, colonization, and nodule development, remain unknown. This understanding requires that the dynamic behavior of gene expression and chromatin accessibility be quantified. Thus, we uncovered the gene regulatory network in response to LCOs in M. truncatula roots by quantifying temporal changes in the transcriptome (RNA-seq) and genome-wide chromatin accessibility (ATAC-seq) after Sinorhizobium meliloti LCO treatment.

Organism:

Medicago truncatula

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL25748 GPL23002

32 Samples

Download data: BED, TXT

(Submitter supplied) Using a dedicated split-root approach, we identified miRNAs regulated systemically by nitrogen availability in both shoots and roots of the Medicago truncatula model legume, depending on the CRA2 pathway, highlighting the phosphate-related miR399.

Organism:

Medicago truncatula

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL30272

24 Samples

Download data: TSV

(Submitter supplied) The involvement of ROS in the legume – Rhizobium symbiotic interaction has been highlighted (Santos et al., 2001; Rubio et al., 2004). This interaction is characterized by the formation of a new organ on the root, the nodule and by the penetration, in parallel, of the bacteria into the root tissue via an infection thread (IT) (Parniske and Downie, 2003; Gage, 2004). H2O2 production has been shown in ITs during the Medicago – Sinorhizobium meliloti interaction (Santos et al., 2001). more...

Organism:

Medicago sativa; Medicago truncatula; Sinorhizobium meliloti

Type:

Genome variation profiling by SNP array

Platform:

GPL4652

8 Samples

Download data: CEL

(Submitter supplied) For transcript analysis of responses in Medicago truncatula to its symbiont Sinorhizobium meliloti wild type or the succinoglycan-deficient exoY mutant we compared transcripts from line A17 roots inoculated with 25 mL OD = 0.05 S. meliloti wild type or exoY mutant. Keywords: 1 line; 2 S. meliloti samples

Organism:

Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4799

6 Samples

Download data

(Submitter supplied) We would like to know how symbiotic molecules such as Nod Factors (NF) influence lateral root (LR) development in M. truncatula. We have preliminary evidence that this action is through early stages of root development. Auxin is the major phytohormone controlling LR development and we also have evidence that NF interfere with auxin for the control of LR development. This transcriptomic study aims at finding new molecular targets that would be responsive to auxin and NF treatment, even at a higher level by the combination of both auxin and NF. more...

Organism:

Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL17428

24 Samples

Download data: PAIR

(Submitter supplied) affy_aphanomyces_medicago - NFP is one of the putative Nod factor receptor and plays therefore a key role in the establishment of symbiosis. nfp allelic mutants are more susceptible to Aphanomyces euteiches (Ae), a root pathogen, than the A17 wild type (WT) line. In this study we want to compare the early plant responses, 1 day post inoculation (dpi) between WT and the nfp2 mutant, in order to identify NFP-dependent gene networks during infection.-Fifteen-day-old A17 or nfp-2 plants were grown in vitro on M medium. more...

Organism:

Sinorhizobium meliloti; Medicago sativa; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4652

12 Samples

Download data: CEL

(Submitter supplied) We have undertaken a detailed study to identify expression polymorphism of NCRs. We used a custom Affymetrix oligonucleotide microarray to examine the expression changes of 566 NCRs in four different accessions of Medicago truncatula. Each accession was inoculated with two different rhizobial strains to assess the NCR expression differences caused by host-symbiont specificity. The NCRs displayed significant expression differences among the ecotypes but had subtle differences in gene expression because of strain difference.

Organism:

Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL14988

24 Samples

Download data: CEL

(Submitter supplied) Medicago truncatula engages in root nodule symbiosis by developing a de novo plant organ (known as nodule) in its roots in response to the infection by rhizobia. These nodules are de novo plant organs that provide an optimal environment for the rhizobia to fix nitrogen in exchange for photosynthates. The establishment of root nodule symbioses (RNS) requires the coordination of two distinct processes: bacterial infection and nodule organogenesis. more...

Organism:

Medicago truncatula

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30272

8 Samples

Download data: MTX, TSV

(Submitter supplied) The dual genome Affymetrix SymbiosisChip contains probe sets for S. meliloti ORFs annotated in 2001, intergenic regions over 150 bp, and ~10,000 probe sets corresponding to expressed sequence tags of the plant host, Medicago truncatula. Protocol: see manufacturer's website

Organism:

Sinorhizobium meliloti; Medicago truncatula

29 Series

275 Samples

Download data: CDF, TXT

(Submitter supplied) Drought is one of the major environmental factors limiting biomass and seed yield production in agriculture. In this research we focused on plants from Fabaceae family, which have a unique ability for establishment of symbiosis with nitrogen-fixing bacteria, and are relatively susceptible to water limitation. We present the changes in nitrogenase activity and global gene expression occurring in Medicago truncatula and Lotus japonicus root nodules during water deficit. more...

Organism:

Mesorhizobium loti; Lotus japonicus; Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by high throughput sequencing

4 related Platforms

16 Samples

Download data: XLSX

(Submitter supplied) The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared transcriptomes of tissue cultures from leaf explants of these two lines. We have used the Affymetrix Medicago Genome Array GeneChip to compare leaf explants of Medicago truncatula Jemalong and the superembryogenic line 2HA after two weeks of tissue culture on 10 µM 1-naphthaleneacetic acid (NAA) and 4 µM 6-benzylaminopurine (BAP) (P4 10:4) Keywords: Genotype comparison

Organism:

Medicago sativa; Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL4652

6 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Medicago truncatula; Arabidopsis thaliana

Type:

Expression profiling by array

Platforms:

GPL14988 GPL14998

87 Samples

Download data: CEL