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| Status |
Public on May 31, 2012 |
| Title |
Gene expression response in roots of Medicago truncatula mutants treated with Myc LCOs |
| Platform organisms |
Sinorhizobium meliloti; Medicago sativa; Medicago truncatula |
| Sample organism |
Medicago truncatula |
| Experiment type |
Expression profiling by array
|
| Summary |
Legumes interact with soil fungi, leading to the development of arbuscular mycorrhizal (AM) roots. Diffusible AM fungal signals were identified as sulphated and non-sulphated LCOs (sMyc-LCOs and nsMyc-LCOs).
Applying Myc-LCOs on roots of symbiotic mutants, we used GeneChips to detail the global programme of gene expression in these mutants in response to the external application of Myc-LCOs.
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| Overall design |
To harvest tissues for transcriptome profiling, three biological replicates consisting of 10 plantlets per treatment were selected. After 6 h of incubation in the climate chamber, 10 plantlets per batch were removed from the treatment (Myc-LCOs) or control solutions and harvested. During harvest, one mm of the root tip of each plantlet was removed and discarded. The remaining 2 to 2.5 cm of the distal root region were cut off and directly frozen in liquid nitrogen.
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| Contributor(s) |
Hohnjec N, Küster H, Czaja LF, Hogekamp C, Maillet F, Becker JD, Dénarié J |
| Citation(s) |
22652128, 26597293 |
| |
| Submission date |
Nov 11, 2011 |
| Last update date |
Nov 25, 2015 |
| Contact name |
Helge Küster |
| E-mail(s) |
[email protected]
|
| Organization name |
Leibniz Universität Hannover
|
| Department |
Institut für Pflanzengenetik
|
| Lab |
Abt. IV - Pflanzengenomforschung
|
| Street address |
Herrenhäuser Str. 2
|
| City |
Hannover |
| ZIP/Postal code |
30419 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL4652 |
[Medicago] Affymetrix Medicago Genome Array |
|
| Samples (24)
|
| GSM831919 |
sMyc-LCOs NFP 6h roots biological replicate 1 |
| GSM831920 |
sMyc-LCOs NFP 6h roots biological replicate 2 |
| GSM831921 |
sMyc-LCOs NFP 6h roots biological replicate 3 |
| GSM831922 |
nsMyc-LCOs NFP 6h roots biological replicate 1 |
| GSM831923 |
nsMyc-LCOs NFP 6h roots biological replicate 2 |
| GSM831924 |
nsMyc-LCOs NFP 6h roots biological replicate 3 |
| GSM831925 |
s/nsMyc-LCOs NFP 6h roots biological replicate 1 |
| GSM831926 |
s/nsMyc-LCOs NFP 6h roots biological replicate 2 |
| GSM831927 |
s/nsMyc-LCOs NFP 6h roots biological replicate 3 |
| GSM831928 |
NFP control 6h roots biological replicate 1 |
| GSM831929 |
NFP control 6h roots biological replicate 2 |
| GSM831930 |
NFP control 6h roots biological replicate 3 |
| GSM831931 |
sMyc-LCOs DMI3 6h roots biological replicate 1 |
| GSM831932 |
sMyc-LCOs DMI3 6h roots biological replicate 2 |
| GSM831933 |
sMyc-LCOs DMI3 6h roots biological replicate 3 |
| GSM831934 |
nsMyc-LCOs DMI3 6h roots biological replicate 1 |
| GSM831935 |
nsMyc-LCOs DMI3 6h roots biological replicate 2 |
| GSM831936 |
nsMyc-LCOs DMI3 6h roots biological replicate 3 |
| GSM831937 |
s/nsMyc-LCOs DMI3 6h roots biological replicate 1 |
| GSM831938 |
s/nsMyc-LCOs DMI3 6h roots biological replicate 2 |
| GSM831939 |
s/nsMyc-LCOs DMI3 6h roots biological replicate 3 |
| GSM831940 |
DMI3 control 6h roots biological replicate 1 |
| GSM831941 |
DMI3 control 6h roots biological replicate 2 |
| GSM831942 |
DMI3 control 6h roots biological replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE33638 |
Medicago truncatula wild-type and mutant roots treated with symbiotic lipochitooligosaccharides |
|
| Relations |
| BioProject |
PRJNA154315 |
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