GEO DataSets

(Submitter supplied) Gene expression profiling of distinct members of a neuronal circuit has the potential to identify candidate molecules and mechanisms that underlie the formation, organization and function of the circuit. To this end, we report here the application of a novel method to characterize RNAs from small numbers of specific Drosophila brain neurons, which belong to the circadian circuit. We identified three different sets of mRNAs enriched in different subclasses of clock neurons: one is enriched in all clock neurons, a second is enriched in PDF-positive clock neurons and a third is enriched in PDF-negative clock neurons. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

23 Samples

Download data: CEL

(Submitter supplied) To compare circadian gene expression within highly discrete neuronal populations, we separately purified and characterized two adjacent but distinct groups of Drosophila adult circadian neurons: the 8 small and 10 large PDF (pigment-dispersing factor)-expressing ventral lateral neurons (s-LNvs and l-LNvs, respectively). The s-LNvs are the principal circadian pacemaker cells, whereas recent evidence indicates that the l-LNvs are involved in sleep and light-mediated arousal. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

24 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platforms:

GPL1322 GPL72

45 Samples

Download data

(Submitter supplied) S2 cells transfected with pAc-Clk or empty vector Keywords: S2 cells Clk

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

6 Samples

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(Submitter supplied) 6 Timepoint microarray from control strain Keywords: Timepoints yw

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

10 Samples

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(Submitter supplied) 6 Timepoints from 5073 strain Keywords: Timepoints 5073 cwo

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

9 Samples

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(Submitter supplied) CLK targets from fly heads using the TIM-GAL4; UAS-CLKGR line Keywords: CLK TARGETS

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platforms:

GPL1322 GPL72

12 Samples

Download data

(Submitter supplied) Experiments performed in S2 cells to identify direct CLK targets Keywords: CLK targets

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platforms:

GPL1322 GPL72

8 Samples

Download data

(Submitter supplied) CWO binding sites were genome-widely searched with Drosophila genome tiling array. Abstract: The Drosophila circadian clock consists of integrated autoregulatory feedback loops, making the clock difficult to elucidate without comprehensively identifying the network components in vivo. Previous studies have adopted genome-wide screening for clock-controlled genes using high-density oligonucleotide arrays that identified hundreds of clock-controlled genes. more...

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL3923

4 Samples

Download data: BED, CEL, ZIP

(Submitter supplied) The circadian clock is an evolutionarily conserved mechanism that drives rhythmic expression of downstream genes. The core circadian clock drives the expression of clock-controlled genes either directly or indirectly, which in turn play critical roles in carrying out many rhythmic physiological processes. Nevertheless, the molecular mechanisms by which clock output genes orchestrate rhythmic signals from the brain to peripheral tissues are largely unknown. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21306

96 Samples

Download data: TXT

(Submitter supplied) LNd, LNv, DN1 and TH neurons were manually sorted from dissociated Drosophila brains. RNA was extracted and transcriptomes analyzed via RNA-seq.

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

48 Samples

Download data: TXT

(Submitter supplied) Single microRNAs are usually associated with hundreds of putative target genes that can influence multiple phenotypic traits in Drosophila, ranging from development to behaviour. We investigated the function of Drosophila miR-210 in circadian behaviour by misexpressing it within circadian clock cells. Manipulation of miR-210 expression levels in the PDF (pigment dispersing factor) positive neurons affected the phase of locomotor activity, under both light-dark conditions and constant darkness. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL18767

16 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNAs) are a novel class of small RNAs which act as modulators of gene expression either by inhibiting the translation or by inducing the degradation of their target mRNAs. Several studies suggest a role for miRNAs as regulators of the circadian clock in mammals and Drosophila. Based on computational predictions of target mRNAs of clock (or clock related) genes, we have selected the miR-210 as a putative regulator of the period clock gene. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL18767

8 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNAs) are a novel class of small RNAs which act as modulators of gene expression either by inhibiting the translation or by inducing the degradation of their target mRNAs. Several studies suggest a role for miRNAs as regulators of the circadian clock in mammals and Drosophila. Based on computational predictions of target mRNAs of clock (or clock related) genes, we have selected the miR-210 as a putative regulator of the period clock gene. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL18767

8 Samples

Download data: TXT

(Submitter supplied) The circadian system produces ~24-hr oscillations in behavioral and physiological processes to ensure that they occur at optimal times of day and in the correct temporal order. At its core, the circadian system is composed of dedicated central clock neurons that keep time through a cell-autonomous molecular clock. To produce rhythmic behaviors, time-of-day information generated by clock neurons must be transmitted across output pathways to regulate the downstream neuronal populations that control the relevant behaviors. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16479

5 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL72

48 Samples

Download data: CEL

(Submitter supplied) Circadian clocks are temporally aligned to the environment via signals, or Zeitgebers, such as daily light and temperature cycles, food availability, and social behavior. In this study, we show that genome-wide expression profiles from temperature-entrained flies show a dramatic difference in the presence or absence of a thermocycle. Whereas transcription appears to be modified globally by changes in temperature, there is a specific set of transcripts that continue to oscillate in constant conditions following temperature entrainment. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL72

12 Samples

Download data: CEL

(Submitter supplied) Circadian clocks are temporally aligned to the environment via signals, or Zeitgebers, such as daily light and temperature cycles, food availability, and social behavior. In this study, we show that genome-wide expression profiles from temperature-entrained flies show a dramatic difference in the presence or absence of a thermocycle. Whereas transcription appears to be modified globally by changes in temperature, there is a specific set of transcripts that continue to oscillate in constant conditions following temperature entrainment. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL72

12 Samples

Download data: CEL

(Submitter supplied) Circadian clocks are temporally aligned to the environment via signals, or Zeitgebers, such as daily light and temperature cycles, food availability, and social behavior. In this study, we show that genome-wide expression profiles from temperature-entrained flies show a dramatic difference in the presence or absence of a thermocycle. Whereas transcription appears to be modified globally by changes in temperature, there is a specific set of transcripts that continue to oscillate in constant conditions following temperature entrainment. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL72

12 Samples

Download data: CEL

(Submitter supplied) Circadian clocks are temporally aligned to the environment via signals, or Zeitgebers, such as daily light and temperature cycles, food availability, and social behavior. In this study, we show that genome-wide expression profiles from temperature-entrained flies show a dramatic difference in the presence or absence of a thermocycle. Whereas transcription appears to be modified globally by changes in temperature, there is a specific set of transcripts that continue to oscillate in constant conditions following temperature entrainment. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL72

12 Samples

Download data: CEL